Traina Evelyn, Daher Silvia, Franchim Camila Sommerauer, Fuziy Juliana Aoki, Moron Antônio Fernandes, Banzato Priscilla Chamelete Andrade, Mattar Rosiane
Departamento de Obstetrícia, Universidade Federal de São Paulo, São Paulo.
Rev Bras Ginecol Obstet. 2010 May;32(5):229-33.
To assess a possible association between polymorphism of the progesterone receptor gene (PROGINS) and recurrent spontaneous abortion (RSA).
In this case-control study, 85 women with at least three previous spontaneous abortions without an identifiable cause (RSA Group) and 157 women with at least two previous term pregnancies without pathologies and no previous miscarriage (Control Group) were selected. An amount of 10 mL of peripheral blood was collected by venipuncture and genomic DNA was extracted by the DTAB/CTAB method, followed by the polymerase chain reaction (PCR) under specific conditions for this polymorphism and by amplification by 2% agarose gel electrophoresis. The bands were visualized with an ultraviolet light transilluminator and the gels were photographed. Differences in the PROGINS genotype and allele frequencies between groups were analyzed by the χ² test, with the level of significance set at p<0.05. The Odds Ratio (OR) was also used, with 95% confidence intervals 95%CI.
PROGINS genotypic frequencies were 72.3% T1T1 and 27.7% T1T2 for the RSA group and 76.4% T1T1, 22.3% T1T2 and 1.3% T2T2 for the control group. There were no differences between groups when the genotype and allele frequencies were analyzed: respectively p=0.48 (OR: 0.8) and p=0.65 (OR: 0.9).
Our results suggest that PROGINS polymorphism is not associated with RSA.
评估孕激素受体基因多态性（PROGINS）与复发性自然流产（RSA）之间可能存在的关联。
在这项病例对照研究中，选取了85名既往至少有三次不明原因自然流产的女性（RSA组）和157名既往至少有两次足月妊娠且无病理情况且无既往流产史的女性（对照组）。通过静脉穿刺采集10 mL外周血，采用DTAB/CTAB法提取基因组DNA，然后在针对该多态性的特定条件下进行聚合酶链反应（PCR），并通过2%琼脂糖凝胶电泳进行扩增。用紫外光透射仪观察条带，对凝胶进行拍照。采用χ²检验分析两组之间PROGINS基因型和等位基因频率的差异，显著性水平设定为p<0.05。还使用了优势比（OR）及95%置信区间（95%CI）。
RSA组PROGINS基因型频率为T1T1占72.3%，T1T2占27.7%；对照组为T1T1占76.4%，T1T2占22.3%，T2T2占1.3%。分析基因型和等位基因频率时，两组之间无差异：p值分别为0.48（OR：0.8）和0.65（OR：0.9）。
我们的结果表明，PROGINS多态性与RSA无关。
