Obstetrics Departament, Universidade Federal de São Paulo, São Paulo, Brazil.
J Reprod Immunol. 2011 Jan;88(1):53-7. doi: 10.1016/j.jri.2010.07.006. Epub 2010 Oct 16.
Hormonal, inflammatory and vascular alterations during pregnancy are thought to be involved in pregnancy loss. The role of progesterone, the actions of which involve cytokines and vascular endothelial growth factor (VEGF), has been thoroughly studied. Genetic factors are involved in modulation of these molecules, and several genes have been associated with recurrent spontaneous abortion (RSA), as well other obstetric conditions. Here we investigate the relationship between RSA and gene polymorphisms of the progesterone receptor (PROGINS), interleukin-1 receptor 1 (PstI) and vascular endothelial growth factor (VEGF) (-634, 936). This case-controlled study comprised a total of 89 women with idiopathic RSA and a control group of 191 women with at least two successful pregnancies and no miscarriages. Genomic DNA was extracted from whole blood, and polymorphism genotyping was conducted by digesting PCR products with specific restriction endonucleases. No correlations were found in any of the investigated polymorphisms, even among dominant, co-dominant and additive inheritance models and alleles. Our results support the hypothesis that these specific gene polymorphisms are not the major determinant of pregnancy success. Although we did not find any correlations, the investigation of these and other polymorphisms remains a challenge in the evaluation of RSA.
人们认为，妊娠期间的激素、炎症和血管改变与妊娠丢失有关。孕激素的作用涉及细胞因子和血管内皮生长因子（VEGF），其作用已得到深入研究。遗传因素参与这些分子的调节，一些基因与复发性自然流产（RSA）以及其他产科疾病有关。在这里，我们研究了 RSA 与孕激素受体（PROGINS）、白细胞介素-1 受体 1（PstI）和血管内皮生长因子（VEGF）（-634、936）基因多态性之间的关系。这项病例对照研究共包括 89 名特发性 RSA 妇女和 191 名至少有两次成功妊娠且无流产史的对照组妇女。从全血中提取基因组 DNA，并通过用特定的限制性内切酶消化 PCR 产物来进行多态性基因分型。在任何研究的多态性中，甚至在显性、共显性和加性遗传模型和等位基因中，都没有发现相关性。我们的结果支持以下假设，即这些特定的基因多态性不是妊娠成功的主要决定因素。尽管我们没有发现任何相关性，但对这些和其他多态性的研究仍然是评估 RSA 的一个挑战。
