Laboratory of Virology, São José do Rio Preto Medical School, São José do Rio Preto, SP, Brazil.
Rev Soc Bras Med Trop. 2010 Sep-Oct;43(5):508-11. doi: 10.1590/s0037-86822010000500007.
The Amazon region has extensive forested areas and natural ecosystems, providing favorable conditions for the existence of innumerous arboviruses. Over 200 arboviruses have been isolated in Brazil and about 40 are associated with human disease. Four out of 40 are considered to be of public health importance in Brazil: Dengue viruses (1-4), Oropouche, Mayaro and Yellow Fever. Along with these viruses, about 98% of the malaria cases are restricted to the Legal Amazon region.
This study aimed to investigate the presence of arboviruses in 111 clinical serum samples from patients living in Novo Repartimento (Pará), Plácido de Castro (Acre), Porto Velho (Rondônia) and Oiapoque (Amapá). The viral RNA was extracted and RT-PCR was performed followed by a Multiplex-Nested-PCR, using Flavivirus, Alphavirus and Orthobunyavirus generic and species-specific primers.
Dengue virus serotype 2 was detected in two patients living in Novo Repartimento (Pará) that also presented active Plasmodium vivax infection.
Despite scant data, this situation is likely to occur more frequently than detected in the Amazon region. Finally, it is important to remember that both diseases have similar clinical findings, thus the diagnosis could be made concomitantly for dengue and malaria in patients living or returning from areas where both diseases are endemic or during dengue outbreaks.
亚马逊地区拥有广阔的森林覆盖区和自然生态系统，为无数虫媒病毒的存在提供了有利条件。在巴西已经分离出超过 200 种虫媒病毒，其中约 40 种与人类疾病有关。在巴西，有 4 种虫媒病毒被认为具有公共卫生重要性：登革热病毒（1-4 型）、奥罗普切病毒、马雅罗病毒和黄热病病毒。除了这些病毒，大约 98%的疟疾病例都局限于法定亚马逊地区。
本研究旨在调查来自 Novo Repartimento（帕拉州）、Plácido de Castro（阿克里州）、波多韦柳（朗多尼亚州）和奥亚波克（阿马帕州）的 111 例临床血清样本中是否存在虫媒病毒。提取病毒 RNA，进行 RT-PCR，然后使用黄病毒、甲病毒和布尼亚病毒通用和种特异性引物进行多重巢式 PCR。
在来自 Novo Repartimento（帕拉州）的两名患者中检测到登革热病毒 2 型，他们同时存在活性间日疟原虫感染。
尽管数据稀少，但这种情况在亚马逊地区可能比检测到的更为频繁。最后，值得记住的是，这两种疾病具有相似的临床特征，因此在患者居住或从疟疾和登革热流行地区返回时，同时进行登革热和疟疾的诊断是很重要的。
