Vieira Deusilene Souza, Honda Eduardo Rezende, Pereira Soraya Santos, Bifano Glauciane da Silva, Tada Mauro Shugiro, Batista Weber Cheli
Instituto de Pesquisa em Patologias Tropicais, Porto Velho, RO, Brasil.
Rev Soc Bras Med Trop. 2007 May-Jun;40(3):268-71. doi: 10.1590/s0037-86822007000300003.
The first dengue fever epidemic in the State of Rondônia (western region of Brazil) was recorded in 1997, without laboratory confirmation. Following this, there was an epidemic in Manaus, in the neighboring State of Amazon, in 1998, in which DENV-1 and DENV-2 viruses were isolated from patients. In the present paper, the serotype characterization of the dengue virus isolated from patients with clinically suspected dengue in Porto Velho, Rondônia, between 2001 and 2003 is described. One hundred and fifty blood samples were collected between the first and fifth days of symptoms. Seventy samples of virus isolates were subjected to dengue identification by means of RT-PCR using universal primers for the NS1 gene of DENV, which amplifies a 419 bp fragment. The amplicons obtained were subjected to enzymatic digestion to characterize the viral serotypes. All the samples analyzed were DENV-1. A nucleotide sequence randomly selected from one amplicon, which was also DENV-1, presented 98% similarity to sequences from Southeast Asia that were obtained from GenBank.
朗多尼亚州（巴西西部地区）的首次登革热疫情于1997年记录在案，但未经实验室确诊。此后，1998年，邻国亚马逊州的马瑙斯发生了疫情，从患者身上分离出了登革热病毒1型（DENV-1）和登革热病毒2型（DENV-2）。在本文中，描述了2001年至2003年期间从朗多尼亚州波多韦柳临床疑似登革热患者中分离出的登革热病毒的血清型特征。在症状出现的第一天至第五天收集了150份血样。使用针对登革热病毒NS1基因的通用引物通过逆转录聚合酶链反应（RT-PCR）对70份病毒分离株样本进行登革热鉴定，该引物可扩增出一个419 bp的片段。对获得的扩增子进行酶切以鉴定病毒血清型。所有分析样本均为DENV-1。从一个同样为DENV-1的扩增子中随机选择的一个核苷酸序列与从GenBank获得的东南亚序列具有98%的相似性。
