Molecular Biology and Research Section, Hospital de Tortosa Verge de la Cinta, IISPV, URV, c/Esplanetes 14, 43500, Tortosa, Spain.
Virchows Arch. 2011 Feb;458(2):237-45. doi: 10.1007/s00428-010-1008-3. Epub 2010 Nov 18.
The Joint Photographic Experts Group (JPEG) standard format is one of the most widely used in image compression technologies. More recently, JPEG2000 format has emerged as a state-of-the-art technology that provides substantial improvements in picture quality at higher compression ratios. However, there has been no attempt to date to determine which of the two compression formats produces less variability in the automated evaluation of immunohistochemically stained digital images in agreement with their compression rates and complexity degrees. The evaluation of Ki67 and FOXP3 immunohistochemical nuclear markers was performed in a total of 329 digital images: 47 were captured in uncompressed Tagged Image File Format (TIFF), 141 were converted to three JPEG compressed formats (47 each with 1:3, 1:23 and 1:46 compression) and 141 were converted to three JPEG2000 compressed formats (47 each with 1:3, 1:23 and 1:46 compression). The count differences between images in TIFF versus JPEG formats were compared with those obtained between images in TIFF versus JPEG2000 formats at the three levels of compression. It was found that, using JPEG2000 compression, the results of the stained nuclei count are close enough to the results obtained with uncompressed images, especially in highly complex images at minimum and medium compression. Otherwise, in images of low complexity, JPEG and JPEG2000 had similar count efficiency to that of the original TIFF images at all compression levels. These data suggest that JPEG2000 could give rise to an efficient means of storage, reducing file size and storage capacity, without compromise on the immunohistochemical analytical quality.
联合图像专家组（JPEG）标准格式是图像压缩技术中应用最广泛的格式之一。最近，JPEG2000 格式已成为一种最先进的技术，在更高的压缩比下提供了显著的图像质量改进。然而，迄今为止，尚未有人试图确定这两种压缩格式中的哪一种在自动评估免疫组织化学染色的数字图像时，在与它们的压缩率和复杂度程度相匹配的情况下，产生的变化性更小。总共对 329 张数字图像进行了 Ki67 和 FOXP3 免疫组化核标记的评估：47 张以未压缩的 Tagged Image File Format (TIFF) 格式捕获，141 张转换为三种 JPEG 压缩格式（47 张分别以 1:3、1:23 和 1:46 压缩），141 张转换为三种 JPEG2000 压缩格式（47 张分别以 1:3、1:23 和 1:46 压缩）。在三个压缩级别下，比较了 TIFF 与 JPEG 格式之间的图像计数差异与 TIFF 与 JPEG2000 格式之间的图像计数差异。结果发现，使用 JPEG2000 压缩时，染色核计数的结果与未压缩图像的结果足够接近，尤其是在最低和中等压缩的高度复杂图像中。否则，在复杂度较低的图像中，JPEG 和 JPEG2000 在所有压缩级别下对原始 TIFF 图像的计数效率相似。这些数据表明，JPEG2000 可以成为一种有效的存储方式，在不影响免疫组织化学分析质量的情况下，减小文件大小和存储容量。
