Departamento de Ciências Morfofisiológicas, Laboratório de Fisiologia da Conservação, Pontifícia Universidade Católica do Rio Grande do Sul, PUCRS, Av. Ipiranga, 6681 Pd. 12A Sala 270, Caixa Postal 1429, Porto Alegre, RS, 90619-900, Brazil.
Ecotoxicology. 2011 Jan;20(1):255-63. doi: 10.1007/s10646-010-0577-x. Epub 2010 Nov 18.
Roundup(®) (glyphosate formulation) is a nonselective and posts emergent herbicide used for controlling aquatic weeds and different concentrations are used in cultures around the world. The objective of this investigation was to examine the effects of Roundup(®) (glyphosate formulation) on the biochemical composition, levels of lipoperoxidation, Na(+)/K(+)ATPase activity and reproductive traits in the Hyalella castroi. Amphipods were collected in summer 2009, in the southern Brazilian highlands. In the laboratory, the animals were kept in aquariums under controlled conditions for 7 days, and after this period they were exposed to 0.36, 0.52, 1.08 and 2.16 mg/l of glyphosate for 7 days. After the period of exposure, the animals were immediately frozen for determination of glycogen, proteins, lipids, triglycerides, cholesterol, levels of lipoperoxidation, and Na(+)/K(+)ATPase activity. During each day of the cultivation reproductive traits (number of reproductive pairs, ovigerous females and eggs in the marsupium) were observed. All concentrations of Roundup(®) induced significant decreases in all biochemical parameters and Na(+)/K(+)ATPase activity, and significant increase in lipoperoxidation levels. Showing this form a potentially toxic effect at very low concentrations, this pattern of results can lead to significant changes in trophic structure of limnic environments because these amphipods are important links in food chain in these habitats.
敌草快（®）（草甘膦制剂）是一种非选择性的、茎叶后除草剂，用于控制水生杂草，世界各地的不同文化中都使用不同浓度的敌草快（®）（草甘膦制剂）。本研究的目的是检测敌草快（®）（草甘膦制剂）对沼泽红点鲑生化成分、脂质过氧化水平、Na(+)/K(+)ATP 酶活性和生殖特征的影响。在 2009 年夏天，在巴西南部高原采集了这些片脚类动物。在实验室中，这些动物在水族箱中处于控制条件下饲养 7 天，在这段时间之后，它们暴露于 0.36、0.52、1.08 和 2.16 mg/L 的草甘膦中 7 天。暴露期结束后，立即将动物冷冻，用于测定糖原、蛋白质、脂质、甘油三酯、胆固醇、脂质过氧化水平和 Na(+)/K(+)ATP 酶活性。在培养期间的每一天，都观察了生殖特征（生殖对的数量、有卵的雌性和育幼囊中卵的数量）。敌草快（®）的所有浓度都显著降低了所有生化参数和 Na(+)/K(+)ATP 酶活性，显著增加了脂质过氧化水平。这表明这种形式在非常低的浓度下具有潜在的毒性作用，这种结果模式可能会导致淡水环境的营养结构发生重大变化，因为这些片脚类动物是这些栖息地食物链中的重要环节。
