National Center for Safety Evaluation of Drugs, National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.
World J Gastroenterol. 2010 Nov 21;16(43):5502-9. doi: 10.3748/wjg.v16.i43.5502.
To study the biological and clinical characteristics of transcription factor forkhead box protein 3 (FOXP3) in hepatocellular carcinoma (HCC).
We analyzed the expression and localization of FOXP3 in HCC tissues and cell lines to evaluate its biological features. The relationship between FOXP3 staining and clinical risk factors of HCC was assessed to identify the clinical characteristics of FOXP3 in HCC.
The mRNA and protein expression of FOXP3 were found in some hepatoma cell lines. Immunohistochemical (IHC) analysis of HCC sections revealed that 48% of HCC displayed FOXP3 staining, but we did not find any FOXP3 staining in normal liver tissues and para-tumor tissues. IHC and Confocal analysis showed that the expressions of FOXP3 were mainly present in the nucleus and cytoplasm of tumor cells in tissues or cell lines. In HCC, the distribution of FOXP3 was similar to that of the cirrhosis, but not to the hepatitis B virus. Those findings implicate that FOXP3 staining seems to be associated with the high risk of HCC.
The clinical characteristics of FOXP3 in HCC warrants further studies to explore its functions and roles in the cirrhosis and development of HCC.
研究转录因子叉头框蛋白 3（FOXP3）在肝细胞癌（HCC）中的生物学和临床特征。
我们分析了 FOXP3 在 HCC 组织和细胞系中的表达和定位，以评估其生物学特征。评估 FOXP3 染色与 HCC 临床危险因素之间的关系，以确定 FOXP3 在 HCC 中的临床特征。
在一些肝癌细胞系中发现了 FOXP3 的 mRNA 和蛋白表达。对 HCC 切片的免疫组织化学（IHC）分析显示，48%的 HCC 显示 FOXP3 染色，但我们在正常肝组织和癌旁组织中未发现任何 FOXP3 染色。IHC 和共聚焦分析显示，FOXP3 的表达主要存在于组织或细胞系中肿瘤细胞的核和细胞质中。在 HCC 中，FOXP3 的分布与肝硬化相似，但与乙型肝炎病毒无关。这些发现表明，FOXP3 染色似乎与 HCC 的高风险相关。
FOXP3 在 HCC 中的临床特征需要进一步研究，以探讨其在肝硬化和 HCC 发展中的功能和作用。
