• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

HBx 通过抑制 miR-187-5p 的转录水平促进肝细胞癌的进展。

HBx promotes hepatocellular carcinoma progression by repressing the transcription level of miR-187-5p.

机构信息

College of Life Sciences and Health, Wuhan University of Science and Technology, Wuhan 430081, China.

Hubei Institute of Blood Transfusion, Wuhan Blood Center, Wuhan 430033, China.

出版信息

Aging (Albany NY). 2023 Aug 1;15(15):7533-7550. doi: 10.18632/aging.204921.

DOI:10.18632/aging.204921
PMID:37531206
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10457053/
Abstract

HBV-associated hepatitis B virus x protein (HBx) plays multiple roles in the development of hepatocellular carcinoma. In our prior study, we discovered that miR-187-5p expression was inhibited by HBx. To investigate the underlying molecular mechanism of HBx-mediated miR-187-5p downregulation in hepatocellular carcinoma cells, effects of HBx and miR-187-5p on hepatoma carcinoma cell were observed, as well as their interactions. Through and experiments, we demonstrated that overexpression of miR-187-5p inhibited proliferation, migration, and invasion. Simultaneously, we observed a dysregulation in the expression of miR-187-5p in liver cancer cell lines, which may be attributed to transcriptional inhibition through the E2F1/FoxP3 axis. Additionally, we noted that HBx protein is capable of enhancing the expression of E2F1, a transcription factor that promotes the expression of FoxP3. In conclusion, our results suggest that the inhibitory effect of HBx on miR-187-5p is mediated through the E2F1/FoxP3 axis. As shown in this work, HBx promotes hepatoma carcinoma cell proliferation, migration, and invasion through the E2F1/FoxP3/miR-187 axis. It provides a theoretical basis for finding therapeutic targets that will help clinic treatment for HCC.

摘要

HBV 相关的乙型肝炎病毒 x 蛋白（HBx）在肝细胞癌的发展中发挥多种作用。在我们之前的研究中，我们发现 miR-187-5p 的表达受到 HBx 的抑制。为了研究 HBx 介导的 miR-187-5p 在肝癌细胞中下调的潜在分子机制，观察了 HBx 和 miR-187-5p 对肝癌细胞的影响及其相互作用。通过 和 实验，我们证明了 miR-187-5p 的过表达抑制了增殖、迁移和侵袭。同时，我们观察到肝癌细胞系中 miR-187-5p 的表达失调，这可能归因于通过 E2F1/FoxP3 轴的转录抑制。此外，我们注意到 HBx 蛋白能够增强转录因子 E2F1 的表达，E2F1 促进 FoxP3 的表达。总之，我们的结果表明，HBx 对 miR-187-5p 的抑制作用是通过 E2F1/FoxP3 轴介导的。如本工作所示，HBx 通过 E2F1/FoxP3/miR-187 轴促进肝癌细胞的增殖、迁移和侵袭。它为寻找治疗靶点提供了理论依据，有助于 HCC 的临床治疗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b696/10457053/b151659b763f/aging-15-204921-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b696/10457053/34d1742fa68d/aging-15-204921-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b696/10457053/726a1a5eaa74/aging-15-204921-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b696/10457053/a364974b62f0/aging-15-204921-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b696/10457053/fd91ec7204f5/aging-15-204921-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b696/10457053/980f2b91c51c/aging-15-204921-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b696/10457053/614cdb3be6b1/aging-15-204921-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b696/10457053/5f0a9ff25234/aging-15-204921-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b696/10457053/b151659b763f/aging-15-204921-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b696/10457053/34d1742fa68d/aging-15-204921-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b696/10457053/726a1a5eaa74/aging-15-204921-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b696/10457053/a364974b62f0/aging-15-204921-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b696/10457053/fd91ec7204f5/aging-15-204921-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b696/10457053/980f2b91c51c/aging-15-204921-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b696/10457053/614cdb3be6b1/aging-15-204921-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b696/10457053/5f0a9ff25234/aging-15-204921-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b696/10457053/b151659b763f/aging-15-204921-g008.jpg

相似文献

1
HBx promotes hepatocellular carcinoma progression by repressing the transcription level of miR-187-5p.HBx 通过抑制 miR-187-5p 的转录水平促进肝细胞癌的进展。
Aging (Albany NY). 2023 Aug 1;15(15):7533-7550. doi: 10.18632/aging.204921.
2
Long non-coding RNA F11-AS1 inhibits HBV-related hepatocellular carcinoma progression by regulating NR1I3 via binding to microRNA-211-5p.长链非编码 RNA F11-AS1 通过与 microRNA-211-5p 结合来调控 NR1I3 抑制 HBV 相关肝细胞癌的进展。
J Cell Mol Med. 2020 Jan;24(2):1848-1865. doi: 10.1111/jcmm.14881. Epub 2019 Dec 27.
3
MiR-19a, miR-122 and miR-223 are differentially regulated by hepatitis B virus X protein and involve in cell proliferation in hepatoma cells.微小RNA-19a、微小RNA-122和微小RNA-223受乙型肝炎病毒X蛋白的差异调节，并参与肝癌细胞的细胞增殖。
J Transl Med. 2016 May 5;14(1):122. doi: 10.1186/s12967-016-0888-7.
4
Downregulation of miR-200a-3p induced by hepatitis B Virus X (HBx) Protein promotes cell proliferation and invasion in HBV-infection-associated hepatocarcinoma.乙型肝炎病毒X蛋白（HBx）诱导的miR-200a-3p下调促进HBV感染相关肝癌的细胞增殖和侵袭。
Pathol Res Pract. 2017 Dec;213(12):1464-1469. doi: 10.1016/j.prp.2017.10.020. Epub 2017 Oct 26.
5
Long non-coding RNA NEAT1 promotes hepatocellular carcinoma cell proliferation through the regulation of miR-129-5p-VCP-IκB.长链非编码RNA NEAT1通过调控miR-129-5p-VCP-IκB促进肝癌细胞增殖。
Am J Physiol Gastrointest Liver Physiol. 2017 Aug 1;313(2):G150-G156. doi: 10.1152/ajpgi.00426.2016. Epub 2017 May 19.
6
Hepatitis B virus X protein boosts hepatocellular carcinoma progression by downregulating microRNA-137.乙型肝炎病毒 X 蛋白通过下调 microRNA-137 促进肝细胞癌进展。
Pathol Res Pract. 2020 Jun;216(6):152981. doi: 10.1016/j.prp.2020.152981. Epub 2020 Apr 23.
7
HBX suppresses PTEN to promote the malignant progression of hepatocellular carcinoma through mi-R155 activation.HBX 通过激活 miR-155 抑制 PTEN 促进肝癌的恶性进展。
Ann Hepatol. 2022 May-Jun;27(3):100688. doi: 10.1016/j.aohep.2022.100688. Epub 2022 Feb 20.
8
Hepatitis B virus promotes proliferation and metastasis in male Chinese hepatocellular carcinoma patients through the LEF-1/miR-371a-5p/SRCIN1/pleiotrophin/Slug pathway.乙型肝炎病毒通过 LEF-1/miR-371a-5p/SRCIN1/pleiotrophin/Slug 通路促进中国男性肝癌患者的增殖和转移。
Exp Cell Res. 2018 Sep 1;370(1):174-188. doi: 10.1016/j.yexcr.2018.06.020. Epub 2018 Jun 19.
9
HBx-induced MiR-1269b in NF-κB dependent manner upregulates cell division cycle 40 homolog (CDC40) to promote proliferation and migration in hepatoma cells.乙肝病毒X蛋白（HBx）以依赖核因子κB（NF-κB）的方式诱导微小RNA-1269b（MiR-1269b）上调细胞分裂周期40同源物（CDC40），从而促进肝癌细胞的增殖和迁移。
J Transl Med. 2016 Jun 27;14(1):189. doi: 10.1186/s12967-016-0949-y.
10
MicroRNA-98-5p Inhibits Tumorigenesis of Hepatitis B Virus-Related Hepatocellular Carcinoma by Targeting NF-κB-Inducing Kinase.微小 RNA-98-5p 通过靶向核因子-κB 诱导激酶抑制乙型肝炎病毒相关肝细胞癌的发生。
Yonsei Med J. 2020 Jun;61(6):460-470. doi: 10.3349/ymj.2020.61.6.460.

引用本文的文献

1
Non-coding RNAs as key regulators in hepatitis B virus-related hepatocellular carcinoma.非编码RNA作为乙型肝炎病毒相关肝细胞癌的关键调节因子
Front Immunol. 2025 Jun 23;16:1602252. doi: 10.3389/fimmu.2025.1602252. eCollection 2025.
2
Impact of VSD therapy on surgical outcomes, inflammatory markers, and functional rehabilitation in patients with secondary bone infection following tibial fracture surgery.VSD治疗对胫骨骨折手术后继发骨感染患者手术结局、炎症标志物及功能康复的影响
Front Cell Infect Microbiol. 2025 Jan 17;14:1508424. doi: 10.3389/fcimb.2024.1508424. eCollection 2024.
3
Epigenetic modification of hepatitis B virus infection and related hepatocellular carcinoma.

本文引用的文献

1
Long non-coding RNA IGF2-AS promotes trophoblast cell proliferation, migration, and invasion by regulating miR-520g/N-cadherin axis.长链非编码 RNA IGF2-AS 通过调节 miR-520g/N-钙黏蛋白轴促进滋养细胞增殖、迁移和侵袭。
J Obstet Gynaecol Res. 2021 Sep;47(9):3047-3059. doi: 10.1111/jog.14886. Epub 2021 Jun 9.
2
FOXP3 and Tip60 Structural Interactions Relevant to IPEX Development Lead to Potential Therapeutics to Increase FOXP3 Dependent Suppressor T Cell Functions.与免疫失调、多内分泌腺病、肠病、X连锁综合征（IPEX）发展相关的FOXP3和Tip60结构相互作用导致了增强FOXP3依赖性抑制性T细胞功能的潜在疗法。
Front Pediatr. 2021 Feb 3;9:607292. doi: 10.3389/fped.2021.607292. eCollection 2021.
3
乙型肝炎病毒感染及其相关肝细胞癌的表观遗传修饰。
Virulence. 2024 Dec;15(1):2421231. doi: 10.1080/21505594.2024.2421231. Epub 2024 Nov 20.
4
The oncogenic role of hepatitis B virus X gene in hepatocarcinogenesis: recent updates.乙型肝炎病毒X基因在肝癌发生中的致癌作用：最新进展
Explor Target Antitumor Ther. 2024;5(1):120-134. doi: 10.37349/etat.2024.00209. Epub 2024 Feb 20.
5
Role of hepatitis B virus non-structural protein HBx on HBV replication, interferon signaling, and hepatocarcinogenesis.乙型肝炎病毒非结构蛋白HBx在乙肝病毒复制、干扰素信号传导及肝癌发生中的作用
Front Microbiol. 2023 Dec 21;14:1322892. doi: 10.3389/fmicb.2023.1322892. eCollection 2023.
miRNA-187-5p Regulates Osteoblastic Differentiation of Bone Marrow Mesenchymal Stem Cells in Mice by Targeting ICAM1.
miRNA-187-5p 通过靶向 ICAM1 调节小鼠骨髓间充质干细胞成骨分化。
Biomed Res Int. 2020 Dec 11;2020:6139469. doi: 10.1155/2020/6139469. eCollection 2020.
4
LncRNA JPX overexpressed in oral squamous cell carcinoma drives malignancy via miR-944/CDH2 axis.在口腔鳞状细胞癌中过表达的长非编码 RNA JPX 通过 miR-944/CDH2 轴驱动恶性转化。
Oral Dis. 2021 May;27(4):924-933. doi: 10.1111/odi.13626. Epub 2020 Nov 20.
5
Hepatitis B virus X protein boosts hepatocellular carcinoma progression by downregulating microRNA-137.乙型肝炎病毒 X 蛋白通过下调 microRNA-137 促进肝细胞癌进展。
Pathol Res Pract. 2020 Jun;216(6):152981. doi: 10.1016/j.prp.2020.152981. Epub 2020 Apr 23.
6
Expression profiles of miRNAs in giant cell tumor of bone showed miR-187-5p and miR-1323 can regulate biological functions through inhibiting FRS2.骨巨细胞瘤中miRNA的表达谱显示，miR-187-5p和miR-1323可通过抑制FRS2来调节生物学功能。
Cancer Med. 2020 May;9(9):3163-3173. doi: 10.1002/cam4.2853. Epub 2020 Mar 10.
7
Deciphering miRNAs' Action through miRNA Editing.通过 miRNA 编辑破译 miRNA 的作用。
Int J Mol Sci. 2019 Dec 11;20(24):6249. doi: 10.3390/ijms20246249.
8
The Regulatory Role of MicroRNA in Hepatitis-B Virus-Associated Hepatocellular Carcinoma (HBV-HCC) Pathogenesis.MicroRNA 在乙型肝炎病毒相关性肝细胞癌（HBV-HCC）发病机制中的调控作用。
Cells. 2019 Nov 24;8(12):1504. doi: 10.3390/cells8121504.
9
Hepatitis B virus X protein enhances hepatocarcinogenesis by depressing the targeting of mRNA by -.乙型肝炎病毒X蛋白通过抑制-对mRNA的靶向作用来增强肝癌发生。
Cancer Biol Med. 2019 May;16(2):276-287. doi: 10.20892/j.issn.2095-3941.2018.0283.
10
Down-Regulation of Circular RNA_000926 Attenuates Renal Cell Carcinoma Progression through miRNA-411-Dependent CDH2 Inhibition.环状 RNA_000926 的下调通过 miRNA-411 依赖性 CDH2 抑制来减弱肾细胞癌的进展。
Am J Pathol. 2019 Dec;189(12):2469-2486. doi: 10.1016/j.ajpath.2019.06.016. Epub 2019 Aug 30.