Clarke F, Cock I, Perkins A, Orozco C
Division of Science and Technology, Griffith University, Nathan Qld, Australia.
Biochem Biophys Res Commun. 1990 Mar 16;167(2):535-42. doi: 10.1016/0006-291x(90)92057-7.
Lipoxygenase metabolites have been found to be active in the rosette inhibition assay by inducing increased rosette inhibition titres. Leukotrienes B4, C4, D4 and E4 were identified as possessing this activity. The cycloxygenase products, prostaglandins E2, D2 and F2 alpha had no such activity; however, prostaglandin E2 and to a lesser degree prostaglandin D2 could counteract the activity of the leukotrienes in this assay. The identified leukotrienes are the first characterised molecules known to display activity in the assay. In this respect they mimic the action of early pregnancy serum, an action ascribed to a so called 'early pregnancy factor'.
已发现脂氧合酶代谢产物在玫瑰花结抑制试验中具有活性，可诱导玫瑰花结抑制效价升高。已鉴定出白三烯B4、C4、D4和E4具有这种活性。环氧化酶产物前列腺素E2、D2和F2α没有这种活性；然而，前列腺素E2以及程度较轻的前列腺素D2可在该试验中抵消白三烯的活性。所鉴定出的白三烯是已知在该试验中显示活性的首批特征明确的分子。在这方面，它们模拟了早孕血清的作用，这种作用归因于一种所谓的“早孕因子”。
