Clarke F M, Orozco C, Perkins A V, Cock I
Division of Science and Technology, Griffith University, Nathan, Queensland, Australia.
J Reprod Fertil. 1990 Mar;88(2):459-66. doi: 10.1530/jrf.0.0880459.
Platelet-activating factor (PAF) stimulated mouse spleen cells to release soluble factors (termed S2 factors) which were capable of inducing increased rosette inhibition titres when applied to fresh mouse spleen cells in the rosette inhibition assay. In this ability the S2 factors mimic that of pregnancy serum, an action previously ascribed to 'early pregnancy factor'. The PAF-stimulated production of these S2 factors was not influenced by inhibitors of cyclooxygenase metabolism, but was completely inhibited by the lipoxygenase inhibitors, diethyl carbamazine and nordihydroguaiaretic acid. The S2 factors had a lipid-like character in that they were extractable in organic solvents. The calcium ionophore A23187 also stimulated the production of these factors which may well be products of the lipoxygenase pathway of arachidonic acid metabolism.
血小板活化因子（PAF）刺激小鼠脾细胞释放可溶性因子（称为S2因子），在玫瑰花结抑制试验中，将这些因子应用于新鲜小鼠脾细胞时，它们能够诱导玫瑰花结抑制效价升高。在这种能力方面，S2因子模拟了妊娠血清的作用，这一作用先前归因于“早孕因子”。PAF刺激产生的这些S2因子不受环氧合酶代谢抑制剂的影响，但被脂氧合酶抑制剂二乙氨基甲嗪和去甲二氢愈创木酸完全抑制。S2因子具有类似脂质的特性，因为它们可被有机溶剂萃取。钙离子载体A23187也刺激这些因子的产生，这些因子很可能是花生四烯酸代谢脂氧合酶途径的产物。
