Reddy V Damodara, Padmavathi P, Paramahamsa M, Varadacharyulu N C
Department of Biochemistry, Sri Krishnadevaraya University, Anantapur, AP 515055, India.
Indian J Biochem Biophys. 2010 Feb;47(1):20-5.
The present study was aimed at investigating the ameliorative effect of Emblica (Phyllanthus Emblica L) fruit extract (EFE) against alcohol-induced oxidative changes in plasma biochemical profile in rats. Alcohol administration (5 g/kg body wt/day) for 60 days resulted in significantly (P<0.05) higher levels of plasma nitrite/nitrate (NOx), total bilirubin, creatinine, and abnormalities in lipid and lipoproteins. Moreover, alcohol receiving rats showed significantly (P<0.05) lowered plasma total protein, albumin/globulin (A/G) ratio and uric acid, with no significant change in glucose level. The EFE administration (250 mg/kg body wt/day) to alcohol-administered rats significantly modulated plasma lipids and lipoprotein patterns and also decreased nitrite/nitrate, total bilirubin and creatinine levels. EFE administration to alcohol receiving rats showed a significant (P<0.05) increase in plasma total protein, A/G ratio and uric acid levels. Total cholesterol (r = 0.466), triglycerides (r = 0.574), VLDL-C (r = 0.578), LDL-C (r = 0.225) and total bilirubin (r = 0.419) showed a stronger positive correlation with that of NOx in alcohol-treated rats. The concentration of nitric oxide (NOx) was negatively correlated with HDL-C (r = -0.285) and uric acid (r = 0.392) in alcohol-treated rats. The amelioration of alcohol-induced oxidative stress might be due to the combined effect of phytophenols, such as tannins and flavonoid compounds and vitamin C.
本研究旨在探讨余甘子（Phyllanthus Emblica L）果实提取物（EFE）对酒精诱导的大鼠血浆生化指标氧化变化的改善作用。给予大鼠酒精（5 g/kg体重/天），持续60天，导致血浆亚硝酸盐/硝酸盐（NOx）、总胆红素、肌酐水平显著升高（P<0.05），脂质和脂蛋白出现异常。此外，接受酒精的大鼠血浆总蛋白、白蛋白/球蛋白（A/G）比值和尿酸显著降低（P<0.05），血糖水平无显著变化。给接受酒精的大鼠给予EFE（250 mg/kg体重/天）可显著调节血浆脂质和脂蛋白模式，并降低亚硝酸盐/硝酸盐、总胆红素和肌酐水平。给接受酒精的大鼠给予EFE后，血浆总蛋白、A/G比值和尿酸水平显著升高（P<0.05）。在酒精处理的大鼠中，总胆固醇（r = 0.466）、甘油三酯（r = 0.574）、极低密度脂蛋白胆固醇（VLDL-C，r = 0.578）、低密度脂蛋白胆固醇（LDL-C，r = 0.225）和总胆红素（r = 0.419）与NOx呈较强的正相关。在酒精处理的大鼠中，一氧化氮（NOx）浓度与高密度脂蛋白胆固醇（HDL-C，r = -0.285）和尿酸（r = 0.392）呈负相关。酒精诱导的氧化应激的改善可能归因于植物酚类（如单宁和黄酮类化合物）和维生素C的联合作用。
