Okafor O Y, Erukainure O l, Ajiboye J A, Adejobi R O, Owolabi F O, Kosoko S B
Food Technology Division, Federal Institute of Industrial Research, Oshodi, Nigeria.
Asian Pac J Trop Biomed. 2011 Jan;1(1):12-4. doi: 10.1016/S2221-1691(11)60060-9.
To investigate the ability of the methanolic extract of pineapple peel to modulate alcohol-induced lipid peroxidation, changes in catalase activities and hepatic biochemical marker levels in blood plasma.
Oxidative stress was induced by oral administration of ethanol (20% w/v) at a dosage of 5 mL/kg bw in rats. After 28 days of treatment, the rats were fasted overnight and sacrificed by cervical dislocation. Blood was collected with a 2 mL syringe by cardiac puncture and was centrifuged at 3 000 rpm for 10 min. The plasma was analyzed to evaluate malondialdehyde (MDA), catalase activity, aspartate aminotransferase (AST), alkaline phosphatase (ALP) and alanine aminotransferase (ALT) concentrations.
Administration of alcohol caused a drastic increase (87.74%) in MDA level compared with the control. Pineapple peel extract significantly reduced the MDA level by 60.16% at 2.5 mL/kg bw. Rats fed alcohol only had the highest catalase activity, treatment with pineapple peel extract at 2.5 mL/kg bw however, reduced the activity. Increased AST, ALP and ALT activities were observed in rats fed alcohol only respectively, treatment with pineapple peel extract drastically reduced their activities.
The positive modulation of lipid peroxidation, catalase activities as well as hepatic biomarker levels of blood plasma by the methanolic extract of pineapple peels under alcohol-induced oxidative stress is an indication of its protective ability in the management of alcohol-induced toxicity.
研究菠萝皮甲醇提取物调节酒精诱导的脂质过氧化、过氧化氢酶活性变化及血浆中肝脏生化标志物水平的能力。
通过给大鼠口服剂量为5 mL/kg体重的乙醇（20% w/v）诱导氧化应激。治疗28天后，大鼠禁食过夜，通过颈椎脱臼处死。用2 mL注射器经心脏穿刺采血，并以3000 rpm离心10分钟。分析血浆以评估丙二醛（MDA）、过氧化氢酶活性、天冬氨酸转氨酶（AST）、碱性磷酸酶（ALP）和丙氨酸转氨酶（ALT）浓度。
与对照组相比，给予酒精导致MDA水平急剧升高（87.74%）。菠萝皮提取物在2.5 mL/kg体重时显著降低了MDA水平，降低了60.16%。仅喂食酒精的大鼠过氧化氢酶活性最高，然而，用2.5 mL/kg体重的菠萝皮提取物处理降低了该活性。仅喂食酒精的大鼠分别观察到AST、ALP和ALT活性增加，用菠萝皮提取物处理显著降低了它们的活性。
在酒精诱导的氧化应激下，菠萝皮甲醇提取物对脂质过氧化、过氧化氢酶活性以及血浆中肝脏生物标志物水平的正向调节表明其在管理酒精诱导的毒性方面具有保护能力。
