NMR Research Centre, Timarpur, Delhi, India.
Int J Radiat Biol. 2011 Jan;87(1):91-7. doi: 10.3109/09553002.2010.518211. Epub 2010 Nov 19.
Radiation exposure induces change in many biological compounds. It is important to assess the physiological and biochemical response to an absorbed dose of ionising radiation due to intentional or accidental event and to predict medical consequences for medical management. In the present study, nuclear magnetic resonance (NMR) spectroscopy-based metabolic profiling was used in mice serum for identification of radiation-induced changes at metabolite level.
Mice were irradiated with 3, 5 and 8 Gray of γ-radiation dose and serum samples collected at day 1, 3 and 5 post irradiation were analysed by proton nuclear magnetic resonance (¹H NMR) spectroscopy. ¹H NMR spectra of serum were analysed by pattern recognition using principal component analysis.
Irradiated mice serum showed distinct metabonomic phenotypes and revealed dose- and time-dependent clustering of irradiated groups. ¹H NMR spectral analysis exhibited increased lactate, amino acids, choline and lipid signals as well as decreased glucose signals. These findings indicate radiation-induced disturbed energy, lipid and protein metabolism.
The information obtained from this study reflects multiple physiological dysfunctions. The study promises the application of NMR-based metabonomics in the field of radiobiology, for development of metabolic-based markers for screening of risk populations and medical management in these cases.
辐射会引起许多生物化合物的变化。由于故意或意外事件，评估吸收剂量的电离辐射的生理和生化反应以及预测医疗后果对于医疗管理非常重要。在本研究中，基于核磁共振（NMR）光谱的代谢组学用于在小鼠血清中鉴定代谢物水平的辐射诱导变化。
用 3、5 和 8 Gray 的 γ 射线剂量照射小鼠，并在照射后第 1、3 和 5 天收集血清样本，通过质子核磁共振（¹H NMR）光谱进行分析。使用主成分分析通过模式识别对血清的 ¹H NMR 光谱进行分析。
照射后的小鼠血清表现出明显的代谢组学表型，并显示出剂量和时间依赖性的照射组聚类。¹H NMR 光谱分析显示乳酸、氨基酸、胆碱和脂质信号增加，葡萄糖信号降低。这些发现表明辐射引起的能量、脂质和蛋白质代谢紊乱。
本研究获得的信息反映了多种生理功能障碍。该研究有望将基于 NMR 的代谢组学应用于放射生物学领域，为筛选风险人群和这些情况下的医疗管理开发基于代谢的标志物。
