Inst. of Botany, Univ. of Warsaw, Krakowskie Przedmiescie 26/28, PL-00-927, Warszawa, Poland.
Physiol Plant. 1990 Aug;79(4):663-7. doi: 10.1111/j.1399-3054.1990.tb00041.x.
Bean (Phaseolus vulgaris L.) seedlings were cultured on complete or phosphate-deficient nutrient medium. After 14 days of culture on phosphate-deficient medium the visible symptoms of P(i) deficiency were observed only in the shoot, the fresh and dry weights of the roots were slightly higher than in control plants. The decreased P(i) content in the roots had little effect on total respiration rate but had an effect on the level of inhibition of respiration by cyanide. The high resistance of respiration to cyanide observed in P(i) -deficient roots was the result of the suppression of cytochrome path activity and an increased participation of the alternative, cyanide-resistant pathway. The cytochrome pathway activity increased when inorganic phosphate was supplied to P(i) -deficient roots for 1 or 3.5 h. It is speculated that the suppression of cytochrome pathway in P(i) -deficient roots may result from restriction of the phosphorylating capacity or a partial inhibition of cytochrome oxidase activity.
将菜豆幼苗培养在完全或缺磷的营养培养基上。在缺磷培养基上培养 14 天后，只有在地上部分才观察到 P(i)缺乏的可见症状，根的鲜重和干重略高于对照植株。根中 P(i)含量的降低对总呼吸速率影响不大，但对氰化物抑制呼吸的程度有影响。在缺磷根中观察到的呼吸对氰化物的高抗性是由于细胞色素途径活性的抑制和替代的、氰化物抗性途径的增加参与所致。当将无机磷酸盐供应给缺磷根 1 或 3.5 小时时，细胞色素途径的活性增加。推测在缺磷根中细胞色素途径的抑制可能是由于磷酸化能力的限制或细胞色素氧化酶活性的部分抑制所致。
