Trutko S M, Medentsev A G, Akimenko V K
Mikrobiologiia. 1980 Sep-Oct;49(5):657-63.
The effect of deficiency in sulfur, copper and iron in the growth medium on cyanide resistant respiration and cytochrome composition was studied in Pseudomonas aeruginosa and Candida lipolytica. It has been shown that: cyanide resistant respiration was observed at the stationary growth phase when the two microorganisms were cultivated in a complete medium; this respiration was detected already at the phase of decelerated growth in the case of copper deficiency; iron deficiency inhibited cyanide resistant respiration in the bacterium but stimulated its appearance in the yeast; sulfur deficiency inhibited the manifestation of cyanide resistant respiration in the both microorganisms; limitation of the bacterial growth with iron resulted in the accumulation of an iron complex (identical to pyoverdin in its spectral characteristics) in the cultural broth; the deficiency of sulfur, copper and iron inhibited the synthesis of all cytochromes in the bacterium; copper deficiency inhibited only the synthesis of a + a3 in the yeast; iron deficiency inhibited the synthesis of all cytochromes in the yeast; sulfur deficiency had virtually no effect on the content of cytochromes in the yeast. A possible nature of cyanide resistant oxidases in these microorganisms is discussed.
研究了在生长培养基中硫、铜和铁缺乏对铜绿假单胞菌和解脂假丝酵母中抗氰呼吸和细胞色素组成的影响。结果表明：当这两种微生物在完全培养基中培养时，在稳定生长期观察到抗氰呼吸；在铜缺乏的情况下，在生长减速期就已检测到这种呼吸；铁缺乏抑制了细菌中的抗氰呼吸，但促进了其在酵母中的出现；硫缺乏抑制了两种微生物中抗氰呼吸的表现；铁限制细菌生长导致培养肉汤中积累一种铁复合物（其光谱特征与绿脓菌素相同）；硫、铜和铁的缺乏抑制了细菌中所有细胞色素的合成；铜缺乏仅抑制酵母中a + a3的合成；铁缺乏抑制酵母中所有细胞色素的合成；硫缺乏对酵母中细胞色素含量几乎没有影响。讨论了这些微生物中抗氰氧化酶的可能性质。
