Fox A J, Curry A, Rowland P L, Lancaster S, Jones D M, Parsons N J, Cole J A, Smith H
Public Health Laboratory, Withington Hospital, Manchester, U.K.
FEMS Microbiol Lett. 1990 Jan 1;54(1-3):75-80. doi: 10.1016/0378-1097(90)90261-n.
A serum-susceptible, guinea-pig chamber-passaged, laboratory strain (BS4 (agar)) of Neisseria gonorrhoeae was converted to serum resistance by incubation with cytidine 5-monophospho-N-acetyl neuraminic acid (CMP-NANA) and examined by electron microscopy after staining with ruthenium-red. In contrast to serum susceptible gonococci incubated without CMP-NANA, the majority (60-70%) of the serum resistant organisms showed a surface accumulation of polysaccharide. This surface polysaccharide was enhanced on all the resistant gonococci after incubation with fresh human serum. Control susceptible gonococci were devoid of the polysaccharide after incubation with heated human serum. Identical results were obtained with a fresh gonococcal isolate which had lost serum resistance on subculture but which, in common with 3 other isolates, was restored to serum resistance by incubation with CMP-NANA.
一株血清敏感的、经豚鼠体内传代培养的淋病奈瑟菌实验室菌株（BS4（琼脂）），通过与胞苷5 - 单磷酸 - N - 乙酰神经氨酸（CMP - NANA）孵育而转变为血清抗性，并在用钌红染色后通过电子显微镜检查。与未用CMP - NANA孵育的血清敏感淋球菌相比，大多数（60 - 70%）血清抗性菌株显示出多糖在表面的积聚。在用新鲜人血清孵育后，所有抗性淋球菌表面的这种多糖都有所增加。对照的敏感淋球菌在用加热的人血清孵育后则没有这种多糖。对于一个新鲜的淋球菌分离株也得到了相同的结果，该分离株在传代培养后失去了血清抗性，但与其他3个分离株一样，通过与CMP - NANA孵育恢复了血清抗性。
