血清因子增强多形核白细胞介导的肿瘤细胞毒性作用
Enhancement of polymorphonuclear leukocyte-mediated tumor cytotoxicity by serum factor(s).
作者信息
Araki A, Inoue T, Kimura S, Fukase S, Sendo F
机构信息
Department of Parasitology, Yamagata University, School of Medicine.
出版信息
Jpn J Cancer Res. 1990 Jan;81(1):69-78. doi: 10.1111/j.1349-7006.1990.tb02509.x.
It has been reported that beta-1,3-D-glucan isolated from Alcaligenes faecalis (TAK) promoted tumor cytolysis by mouse polymorphonuclear leukocytes (PMN). We investigated the effect of serum on mouse PMN tumor cytolysis induced by TAK and other PMN stimulators. Addition of fetal calf serum (FCS) to the cytolysis assay enhanced tumor cytolysis by PMN in a dose-dependent manner. Sera obtained from horses, mice, and rats were also effective enhancers of PMN tumor cytolysis. When FCS was added after the assay was under way, the enhancing effect decreased proportionally to the time elapsed. The enhancing activity was detected over a broad range of fractions with a peak at 170 kD by fractionation on a Superose 6 column. The responsible factor(s) in serum was stable after treatment at 60 degrees C, 30 min or after lowering the pH to 2. Mouse PMN stimulated with TAK increased production of hydrogen peroxide in the presence of FCS.
据报道，从粪产碱杆菌（TAK）中分离出的β-1,3-D-葡聚糖可促进小鼠多形核白细胞（PMN）的肿瘤细胞溶解。我们研究了血清对TAK及其他PMN刺激剂诱导的小鼠PMN肿瘤细胞溶解的影响。在细胞溶解试验中添加胎牛血清（FCS）可使PMN的肿瘤细胞溶解呈剂量依赖性增强。从马、小鼠和大鼠获得的血清也是PMN肿瘤细胞溶解的有效增强剂。在试验开始后添加FCS时，增强作用会随着时间的推移成比例下降。通过在Superose 6柱上进行分级分离，在广泛的级分范围内检测到增强活性，峰值出现在170 kD处。血清中的相关因子在60℃处理30分钟或pH值降至2后仍保持稳定。在FCS存在的情况下，用TAK刺激的小鼠PMN会增加过氧化氢的产生。
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