大鼠组织DNA中黄曲霉毒素B1和G1加合物水平的细胞间差异：一项定量免疫细胞化学研究。
Intercellular variation in levels of adducts of aflatoxin B1 and G1 in DNA from rat tissues: a quantitative immunocytochemical study.
作者信息
Wild C P, Montesano R, Van Benthem J, Scherer E, Den Engelse L
机构信息
The Netherlands Cancer Institute, Antoni Van Leeuwenhoek Huis, Amsterdam.
出版信息
J Cancer Res Clin Oncol. 1990;116(2):134-40. doi: 10.1007/BF01612667.
Abstract
Adducts between aflatoxin B1 and G1 and DNA have been visualised and quantified in various rat tissues by a sensitive immunocytochemical approach. The quantitative validity of this assay has been examined by comparison with experiments using radioactively labelled aflatoxin. Rats were exposed to single and multiple doses of aflatoxin and a marked intercellular variation in adduct levels was observed in kidney and lung, in contrast to the liver, where binding was more homogeneous. No adducts were detected in the oesophagus, forestomach, colon, spleen or testes (detection limit approximately 300 pg aflatoxin/mg DNA). The DNA adduct data are discussed in relation to the carcinogenicity of aflatoxin B1 and G1.
摘要
通过一种灵敏的免疫细胞化学方法，已在各种大鼠组织中对黄曲霉毒素B1和G1与DNA之间的加合物进行了可视化和定量分析。通过与使用放射性标记黄曲霉毒素的实验进行比较，检验了该检测方法的定量有效性。将大鼠暴露于单剂量和多剂量的黄曲霉毒素中，结果发现，与肝脏中结合更为均匀不同，在肾脏和肺中观察到加合物水平存在明显的细胞间差异。在食管、前胃、结肠、脾脏或睾丸中未检测到加合物（检测限约为300 pg黄曲霉毒素/毫克DNA）。结合黄曲霉毒素B1和G1的致癌性对DNA加合物数据进行了讨论。
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