Buendia B, Bré M H, Griffiths G, Karsenti E
European Molecular Biology Laboratory, Heidelberg, FRG.
J Cell Biol. 1990 Apr;110(4):1123-35. doi: 10.1083/jcb.110.4.1123.
The two centrioles that are localized close to each other and to the nucleus in single Madin-Darby Canine kidney cells (MDCK) move apart by distances as large as 13 microns after the establishment of extensive cellular junctions. Microfilaments, and possibly microtubules appear to be responsible for this separation. In fully polarized cells, the centrioles are localized just beneath the apical membrane. After disruption of intercellular junctions in low calcium medium, the centrioles move back towards the cell center. This process requires intact microtubules but happens even in the absence of microfilaments. These results indicate that the position of centrioles is determined by opposing forces produced by microtubules and microfilaments and suggest that the balance between these forces is modulated by the assembly of cellular junctions. Centriole separation appears to be an early event in the process that precedes their final positioning in the apical-most region of the polarized cell.
在单个犬肾细胞（MDCK）中，两个彼此靠近且靠近细胞核的中心粒，在广泛的细胞连接建立后，会分开达13微米的距离。微丝，可能还有微管，似乎对这种分离负责。在完全极化的细胞中，中心粒位于顶端膜下方。在低钙培养基中破坏细胞间连接后，中心粒会向细胞中心移动。这个过程需要完整的微管，但即使在没有微丝的情况下也会发生。这些结果表明，中心粒的位置由微管和微丝产生的相反力量决定，并表明这些力量之间的平衡受到细胞连接组装的调节。中心粒分离似乎是它们最终定位在极化细胞最顶端区域之前过程中的一个早期事件。
