Smitz J, Bollen N, Camus M, Devroey P, Wisanto A, Van Steirteghem A C
Centre for Reproductive Medicine, Akademisch Ziekenhuis, Vrije Universiteit Brussel, Belgium.
Hum Reprod. 1990 Feb;5(2):157-62. doi: 10.1093/oxfordjournals.humrep.a137061.
Ten endocrinologically normal women were injected subcutaneously with 500 micrograms D-Ser(TBU)6-EA10-LHRH (buserelin) on days 3,4 and 5 after the start of the menses. Two types of response were observed. Five women (group A) responded promptly and had a mean number of 13.4 oocytes retreived after 11.4 days of stimulation. In the second group (B), two to three times more HMG was needed to obtain a mean number of 7.3 oocytes after 17.2 days of stimulation. The response upon stimulation could be predicted by the serum gonadotrophin output on days 4 and 5 of the cycle. One woman from group B had a premature LH rise on day 16 and luteinization; her cycle was abandoned. In the four other patients of group B, serum and urinary LH concentrations showed that pituitary gonadotrophin secretion had recovered before the ovulatory stimulus, without signs of premature luteinization. Two women in each group became pregnant, one of whom aborted. This short-term GnRH agonist treatment could be an alternative method for ovarian stimulation, although it did not totally prevent the occurrence of an endogenous LH surge.
10名内分泌功能正常的女性在月经开始后的第3、4和5天皮下注射500微克D-丝氨酸（叔丁基）6-乙基酰胺10-促性腺激素释放激素（布舍瑞林）。观察到两种反应类型。5名女性（A组）反应迅速，在刺激11.4天后平均获得13.4个回收卵母细胞。在第二组（B组）中，需要两到三倍的人绝经期促性腺激素才能在刺激17.2天后平均获得7.3个卵母细胞。刺激反应可通过周期第4天和第5天的血清促性腺激素输出量来预测。B组的一名女性在第16天出现促黄体生成素过早升高和黄体化；她的周期被放弃。在B组的其他4名患者中，血清和尿促黄体生成素浓度显示，垂体促性腺激素分泌在排卵刺激前已恢复，无过早黄体化迹象。每组有两名女性怀孕，其中一名流产。这种短期促性腺激素释放激素激动剂治疗可能是卵巢刺激的一种替代方法，尽管它不能完全防止内源性促黄体生成素高峰的发生。
