Shi Huai, Liu Bo, Su Mingxing, Huang Sufang, Zhu Yujing
Agricultural Bioresources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China.
Sheng Wu Gong Cheng Xue Bao. 2010 Aug;26(8):1080-7.
The paper dealt with the characterization of polysaccharide of Paecilomyces lilacinus NH-PL-03 strain. First, we extracted and purified exude polysaccharide from the fungal fermentation broth by ethanol depositing method. Second, the proteins were removed by the Sevage method from the crude polysaccharide. Third, the purified polysaccharide (EP-1) was obtained after Superdex G-75 column separation. The results of UV-spectrometer and Sephacryl S-200 HR chromatography experiments showed that the EP-1 was a homogeneous pure polysaccharide with molecular weight of 35.2 kDa. Tested by paper chromatography analysis using the complete hydrolysis by sulfuric acid, we found that the EP-1 comprise single component as glucose. The chemical structure of EP-1 was confirmed as a kind of linear glucan linked by beta-(1,3) linkage. The Congo red reaction performed that EP-1 probable presented a triple-helical conformation in the dilute alkali.
该论文研究了淡紫拟青霉NH-PL-03菌株多糖的特性。首先，我们通过乙醇沉淀法从真菌发酵液中提取并纯化了胞外多糖。其次，采用Sevage法从粗多糖中去除蛋白质。第三，经Superdex G-75柱分离后得到纯化多糖（EP-1）。紫外光谱仪和Sephacryl S-200 HR色谱实验结果表明，EP-1是一种分子量为35.2 kDa的均匀纯多糖。通过硫酸完全水解后的纸层析分析测试，我们发现EP-1由单一成分葡萄糖组成。EP-1的化学结构被确认为一种由β-(1,3)键连接的线性葡聚糖。刚果红反应表明，EP-1在稀碱中可能呈现三螺旋构象。
