Sudha P, Asdaq Syed Mohammed Basheeruddin, Dhamingi Sunil S, Chandrakala Gowda Kallenahalli
Department of Pharmacology, Krupanidhi College of Pharmacy, Bangalore.
Indian J Physiol Pharmacol. 2010 Apr-Jun;54(2):133-40.
The aim of the present study was to investigate the immunomodulatory action of methanolic extract of Moringa oleifera (MEMO) in an experimental model of immunity. The cellular immunity was evaluated using neutrophil adhesion test, cyclophosphamide induced neutropenia and carbon clearance assay, whereas, humoral immunity was tested by mice lethality test, serum immunoglobulin estimation and indirect haemagglutination assay in animals. Administration of MEMO (250 and 750 mg/kg, po) and Ocimum sanctum (100 mg/kg, po) significantly increased the levels of serum immunoglobulins and also prevented the mortality induced by bovine Pasteurella multocida in mice. They also increased significantly the circulating antibody titre in indirect haemagglunation test. Moreover, MEMO produced significant increase in adhesion of neutrophils, attenuation of cyclophosphamide-induced neutropenia and an increase in phagocytic index in carbon clearance assay. From the above results, it can be concluded that MEMO stimulate both cellular and humoral immune response. However, low dose of MEMO was found to be more effective than the high dose.
本研究的目的是在免疫实验模型中研究辣木甲醇提取物（MEMO）的免疫调节作用。通过中性粒细胞黏附试验、环磷酰胺诱导的中性粒细胞减少和碳清除试验评估细胞免疫，而通过小鼠致死试验、血清免疫球蛋白估计和动物间接血凝试验检测体液免疫。给予MEMO（250和750mg/kg，口服）和神圣罗勒（100mg/kg，口服）可显著提高血清免疫球蛋白水平，并预防小鼠感染多杀性巴氏杆菌牛源亚种所致的死亡。它们还显著提高了间接血凝试验中的循环抗体滴度。此外，MEMO在碳清除试验中使中性粒细胞黏附显著增加、环磷酰胺诱导的中性粒细胞减少减轻且吞噬指数增加。从上述结果可以得出结论，MEMO可刺激细胞免疫和体液免疫反应。然而，发现低剂量的MEMO比高剂量更有效。
