辣木叶片甲醇提取物对Wistar白化大鼠的免疫调节活性
Immunomodulatory activity of methanolic leaf extract of Moringa oleifera in Wistar albino rats.
作者信息
Nfambi Joshua, Bbosa Godfrey S, Sembajwe Lawrence Fred, Gakunga James, Kasolo Josephine N
出版信息
J Basic Clin Physiol Pharmacol. 2015 Nov;26(6):603-11. doi: 10.1515/jbcpp-2014-0104.
BACKGROUND
Globally, Moringa oleifera is used by different communities to treat various ailments including modulation of the immune system though with limited scientific evidence. The aim was to study the immunomodulatory activity of M. oleifera methanolic leaf extract in Wistar albino rats.
METHODS
An experimental laboratory-based study was done following standard methods and procedures. Nine experimental groups (I, II, III, IV, V, VI, VII, VIII, IX) each comprising of six animals were used. Group I received normal saline. Groups II to IX received 200 mg/kg bwt cyclophosphamide at the beginning of the study. Group III received 50 mg/kg bwt of an immunostimulatory drug levamisole. Groups IV to IX were dosed daily for 14 days with extract at doses of 250, 500, and 1000 mg/kg bwt, respectively, using an intragastric tube. Complete blood count (CBC), delayed-type hypersensitivity reaction (DTH), neutrophil adhesion test, and hemagglutination antibody titer were determined using standard methods and procedures. Statistical analysis was performed using GraphPad prism 5.0a Software.
RESULTS
There was an increment in WBC, lymphocyte, and neutrophil counts at a dose of 1000 mg/kg bwt similar to the levamisole-positive control group. The neutrophil adhesion was statistically significant (p≤0.05) for treatment groups that received 1000 mg/kg bwt (29.94%) and 500 mg/kg bwt at 17.28%. The mean percentage increment in footpad thickness was highest (26.9%) after 8 h of injection of antigen in the footpad of rats dosed 500 mg/kg bwt and this later reduced to 25.6% after 24 h. There was a dose-dependent increment in the mean hemagglutination antibody titer to sheep red blood cells (SRBC) from 10.73±0.57 HA units/μL for the 250 mg/kg bwt to 26.22±1.70 HA units/μL for the 1000 mg/kg bwt.
CONCLUSIONS
Methanolic leaf extract of M. oleifera caused a significant immunostimulatory effect on both the cell-mediated and humoral immune systems in the Wistar albino rats.
背景
在全球范围内，不同社区使用辣木来治疗各种疾病，包括调节免疫系统，不过相关科学证据有限。本研究旨在探讨辣木甲醇叶提取物对Wistar白化大鼠的免疫调节活性。
方法
按照标准方法和程序进行了一项基于实验室的实验研究。使用了9个实验组（I、II、III、IV、V、VI、VII、VIII、IX），每组6只动物。I组给予生理盐水。II至IX组在研究开始时给予200mg/kg体重的环磷酰胺。III组给予50mg/kg体重的免疫刺激药物左旋咪唑。IV至IX组分别以250、500和1000mg/kg体重的剂量通过胃管每日给药14天。使用标准方法和程序测定全血细胞计数（CBC）、迟发型超敏反应（DTH）、中性粒细胞黏附试验和血凝抗体滴度。使用GraphPad prism 5.0a软件进行统计分析。
结果
剂量为1000mg/kg体重时，白细胞、淋巴细胞和中性粒细胞计数增加，与左旋咪唑阳性对照组相似。接受1000mg/kg体重（29.94%）和500mg/kg体重（17.28%）的治疗组中性粒细胞黏附具有统计学意义（p≤0.05）。在给500mg/kg体重的大鼠足垫注射抗原8小时后，足垫厚度的平均百分比增量最高（26.9%），24小时后降至25.6%。对绵羊红细胞（SRBC）的平均血凝抗体滴度呈剂量依赖性增加，从250mg/kg体重时的10.73±0.57HA单位/μL增加到1000mg/kg体重时的26.22±1.70HA单位/μL。
结论
辣木甲醇叶提取物对Wistar白化大鼠的细胞介导免疫和体液免疫系统均产生了显著的免疫刺激作用。
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