白花丹中裂环烯醚萜类成分的体外和体内免疫调节活性。
In vitro and in vivo immunomodulatory activities of iridoids fraction from Barleria prionitis Linn.
机构信息
Institute of Pharmaceutical Education and Research, Wardha 442 001, Maharashtra State, India.
出版信息
J Ethnopharmacol. 2012 May 7;141(1):424-31. doi: 10.1016/j.jep.2012.03.005. Epub 2012 Mar 13.
ETHNOPHARMACOLOGICAL RELEVANCE
Barleria prionitis Linn. (Family: Acanthaceae), one of the important Ayurvedic medicinal plant in India, has long been used to treat variety of ailments including swellings, gout, arthritic and rheumatic disorders, nervine and skin diseases, and also acts as immunorestorative.
AIM OF THE STUDY
The present study was aimed to explore in vitro and in vivo immunomodulatory activities of the iridoids fraction i.e. n-butanol fraction of methanol extract from Barleria prionitis aerial parts (IFBp).
MATERIALS AND METHODS
IFBp was studied for in vitro [nitroblue tetrazolium (NBT) test and neutrophils candidacidal assay] and in vivo immunomodulatory activity on cellular and humoral immune responses to the antigenic challenge by sheep red blood cells (SRBCs) and by neutrophil adhesion test, phagocytic activity and cyclophosphamide-induced myelosuppression. The study comprised the preliminary phytochemical screening, HPTLC standardization and maximum tolerable dose determination of IFBp.
RESULTS
IFBp (50, 100 and 200μg/ml) significantly (P≤0.01) increased the intracellular killing activity of stimulated neutrophils assayed by in vitro NBT reduction test and neutrophils candidacidal assay. Pretreatment of IFBp (100 and 200mg/kg; p.o.) evoked a significant increase in percent neutrophils and neutrophils adhesion to nylon fibres. Oral administration of IFBp augmented the humoral immune response to SRBCs, evidenced by increase in antibody titres and dose dependently potentiated the delayed-type hypersensitivity reaction induced by SRBCs in mice. IFBp potentiated significantly (P≤0.01) the macrophage phagocytic activity and ameliorated the red blood cells, total white blood cells and platelets count and haemoglobin concentration, and also restored the myelosuppressive effects induced by cyclophosphamide. The content (% w/w; mean±SD, n=3) of main iridoids i.e. shanzhiside methyl ester and barlerin was found to be 21.55±2.40 and 10.03±1.69 in IFBp of BP, respectively.
CONCLUSION
The present investigation reveals that IFBp is a potent immunostimulant, stimulating both the specific and non-specific immune mechanisms.
民族药理学相关性
巴尔勒里亚·普里奥尼斯·林恩（家族：爵床科），印度重要的阿育吠陀药用植物之一，长期以来一直用于治疗各种疾病，包括肿胀、痛风、关节炎和风湿病、神经和皮肤疾病，并且还具有免疫恢复作用。
研究目的
本研究旨在探索巴尔勒里亚·普里奥尼斯地上部分甲醇提取物的正丁醇部分（IFBp）中的环烯醚萜类化合物的体外和体内免疫调节活性。
材料和方法
IFBp 进行了体外 [硝基四唑蓝 (NBT) 试验和中性粒细胞杀菌试验] 和体内免疫调节活性研究，以研究抗原性挑战对细胞和体液免疫的影响，抗原性挑战由绵羊红细胞 (SRBC) 引起，并通过中性粒细胞黏附试验、吞噬活性和环磷酰胺诱导的骨髓抑制来研究。该研究包括 IFBp 的初步植物化学筛选、HPTLC 标准化和最大耐受剂量确定。
结果
IFBp（50、100 和 200μg/ml）显著（P≤0.01）增加了体外 NBT 还原试验和中性粒细胞杀菌试验测定的刺激中性粒细胞的细胞内杀伤活性。IFBp（100 和 200mg/kg；po）预处理可引起中性粒细胞百分比和中性粒细胞黏附到尼龙纤维的显著增加。IFBp 的口服给药增强了对 SRBC 的体液免疫反应，表现为抗体滴度增加，并呈剂量依赖性增强 SRBC 诱导的迟发型超敏反应。IFBp 显著增强（P≤0.01）巨噬细胞吞噬活性，并改善红细胞、总白细胞和血小板计数以及血红蛋白浓度，并恢复环磷酰胺诱导的骨髓抑制作用。IFBp 中主要环烯醚萜类化合物（即山栀苷甲酯和巴尔莱林）的含量（%w/w；平均值±SD，n=3）分别为 21.55±2.40 和 10.03±1.69。
结论
本研究表明，IFBp 是一种有效的免疫刺激剂，刺激特异性和非特异性免疫机制。
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