State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, The Chinese Academy of Sciences, Wuhan 430071, China.
J Am Chem Soc. 2010 Dec 15;132(49):17349-51. doi: 10.1021/ja107745g. Epub 2010 Nov 19.
The feasibility of a (1)H-(14)N HSQC experiment on tissues is demonstrated with a mouse liver based on the J couplings between the protons and the quadrupolar nucleus (14)N in choline. Free choline, phosphocholine, and glycerolphosphocholine (1)H-(14)N HSQC signals were selectively observed with all unwanted signals cleanly suppressed. The CH2O signals were well resolved in the two-dimensional spectrum, which can be used for quantitative analyses.
本文基于胆碱中质子与四极核（14）N 之间的 J 耦合，证明了在鼠肝上进行（1）H-（14）N HSQC 实验的可行性。通过选择性地观察游离胆碱、磷酸胆碱和甘油磷酸胆碱的（1）H-（14）N HSQC 信号，同时完全抑制所有不需要的信号，实现了（1）H-（14）N HSQC 信号的选择性观测。二维谱中 CH2O 信号具有良好的分辨率，可用于定量分析。
