Department of Chemistry, Institute of BioPhysio Sensor Technology, Pusan National University, Busan 609-735, Korea.
Anal Chem. 2010 Dec 15;82(24):10075-82. doi: 10.1021/ac102041u. Epub 2010 Nov 19.
A peroxynitrite (ONOO(-)) biosensor has been developed through the preparation of a new manganese-[poly-2,5-di-(2-thienyl)-1H-pyrrole)-1-(p-benzoic acid)] (Mn-pDPB) complex. DPB monomer was first synthesized and polymerized for the purpose of providing a polymer backbone for complex formation with Mn(2+) ion. The Mn-pDPB complex was characterized via Magnetomotive Force (MMF) simulation, X-ray photoelectron spectroscopy (XPS), and cyclic voltammetry. The complex selectively enhanced the reduction process of ONOO(-) which was used as the analytical signal for chronoamperometric detection. A polyethyleneimmine (PEI) layer was coated on the complex surface to increase selectivity and stability. The chronoamperometric calibration plot showed the hydrodynamic range of 2.0 × 10(-8)-5.0 × 10(-7) M. The detection limit was determined to be 1.9 (±0.2) × 10(-9) M based on S/N = 3. The microbiosensor, fabricated on a 100 μm diameter Pt tip, was applied in a real rat plasma sample for the detection of spiked concentrations of ONOO(-). The reliability and long-term stability of the microbiosensor was also examined with YPEN-1 cells in vitro, and the results shown were promising.
一种过氧亚硝酸盐（ONOO(-)）生物传感器已经通过制备一种新的锰-[聚-2,5-二-(2-噻吩基)-1H-吡咯)-1-(对苯甲酸)]（Mn-pDPB）配合物而开发出来。DPB 单体首先被合成并聚合，目的是为 Mn(2+)离子的配合物形成提供聚合物主链。Mn-pDPB 配合物通过磁动势（MMF）模拟、X 射线光电子能谱（XPS）和循环伏安法进行了表征。该配合物选择性地增强了 ONOO(-)的还原过程，该过程被用作计时安培检测的分析信号。在复合物表面涂覆一层聚乙烯亚胺（PEI）层，以提高选择性和稳定性。计时安培校准曲线显示出 2.0×10(-8)-5.0×10(-7)M 的水动力范围。根据 S/N = 3，检测限确定为 1.9（±0.2）×10(-9)M。该微生物传感器在 100 μm 直径的 Pt 尖端上制造，并应用于实际大鼠血浆样品中，以检测 ONOO(-)的加标浓度。微生物传感器的可靠性和长期稳定性也在体外的 YPEN-1 细胞中进行了检查，结果令人鼓舞。
