Department of Chemistry and Institute of BioPhysio Sensor Technology, Pusan National University, Busan 609-735, Republic of Korea.
Biomaterials. 2012 Mar;33(9):2600-7. doi: 10.1016/j.biomaterials.2011.12.026. Epub 2011 Dec 30.
A highly sensitive in vivo biosensor for glutathione disulfide (GSSG) is developed using covalently immobilized-glutathione reductase (GR) and -β-nicotinamide adenine dinucleotide phosphate (NADPH) on gold nanoparticles deposited on poly[2,2':5',2″-terthiophene-3'-(p-benzoic acid)] (polyTTBA). The fabricated biosensor was characterized with SEM, TEM, XPS, and QCM. Analytical parameters affecting the biosensor performance were optimized in terms of applied potential, NADPH:GR ratio, temperature, and pH. A linear calibration plot is obtained using chronoamperometry in the dynamic range between 0.1 μM and 2.5 mM of GSSG, with a detection limit of 12.5 ± 0.5 nM. The developed biosensor is applied to detect GSSG in a real plasma sample. A microbiosensor was applied to detect the in vivo GSSG concentration to monitor the oxidative stress caused by diquat and t-butyl hydroperoxide. The results obtained are reliable, implying a promising approach for a GSSG biosensor in clinical diagnostics and oxidative stress monitoring.
一种高灵敏度的体内生物传感器用于谷胱甘肽二硫化物（GSSG）是使用共价固定化谷胱甘肽还原酶（GR）和β-烟酰胺腺嘌呤二核苷酸磷酸（NADPH）在金纳米粒子沉积在聚[2,2':5',2″-三噻吩-3'-(对苯甲酸)]（polyTTBA）上开发的。所制备的生物传感器通过 SEM、TEM、XPS 和 QCM 进行了表征。从应用电位、NADPH:GR 比、温度和 pH 等方面优化了影响生物传感器性能的分析参数。使用计时安培法在 GSSG 的动态范围内（0.1 μM 至 2.5 mM）获得线性校准图，检测限为 12.5 ± 0.5 nM。该开发的生物传感器用于检测真实血浆样品中的 GSSG。微生物传感器用于检测体内 GSSG 浓度以监测百草枯和叔丁基过氧化物引起的氧化应激。所获得的结果是可靠的，这意味着在临床诊断和氧化应激监测中用于 GSSG 生物传感器的有前途的方法。
