RIKEN Advanced Science Institute, Wako, Saitama 351-0198, Japan.
Chem Soc Rev. 2011 Dec;40(12):5815-28. doi: 10.1039/c1cs15025a. Epub 2011 Jun 10.
An excitonic interaction caused by the H-aggregation of fluorescent dyes is a new type of useful photophysical process for fluorescence-controlled nucleic acid sensing. This critical review points out the recent advances in exciton-controlled hybridization-sensitive fluorescent oligonucleotide (ECHO) probes, which have a fluorescence-labeled nucleotide in which two molecules of thiazole orange or its derivatives are linked covalently. ECHO probes show absorption shift and emission switching depending on hybridization with the target nucleic acid. The hybridization-sensitive fluorescence emission of ECHO probes and the further modification of probes have made possible a variety of practical applications, such as multicolor RNA imaging in living cells and facile detection of gene polymorphism (144 references).
荧光染料的 H 聚集引起的激子相互作用是一种用于控制荧光的核酸传感的新型有用光物理过程。这篇评论性文章指出了激子控制杂交敏感荧光寡核苷酸（ECHO）探针的最新进展，ECHO 探针在荧光标记核苷酸中有两个噻唑橙或其衍生物分子通过共价键连接。ECHO 探针的吸收位移和发射切换取决于与靶核酸的杂交。ECHO 探针的杂交敏感荧光发射以及探针的进一步修饰使得多种实际应用成为可能，例如活细胞中多色 RNA 成像和基因多态性的简便检测（引用 144 篇）。
