Pankowsky D A, Ziats N P, Topham N S, Ratnoff O D, Anderson J M
Institute of Pathology, Case Western Reserve University, Cleveland, OH 44106.
J Vasc Surg. 1990 Apr;11(4):599-606.
Protein adsorption on the surfaces of clinically significant prosthetic vascular graft materials from human whole blood was independent of plasma concentration as determined morphologically by use of immunogold labels. Some proteins, such as fibrinogen, adsorbed in a multilayer pattern on expanded polytetrafluoroethylene and had a preference for particular surface features of the polymer. Other proteins, such as Hageman factor (factor XII), showed diffuse adsorption patterns. Physiologically significant proteins that have not been well studied, such as immunoglobulin G and factor VIII, adsorbed readily to the surface of expanded polytetrafluoroethylene. This finding may be significant since adsorbed proteins may activate coagulation mechanisms and immunologic responses, including platelet and monocyte adhesion and activation. Any human blood protein for which an antibody has been developed can be studied by use of this technique.
利用免疫金标记进行形态学测定发现，来自人全血的临床上重要的人工血管移植材料表面的蛋白质吸附与血浆浓度无关。一些蛋白质，如纤维蛋白原，以多层模式吸附在膨体聚四氟乙烯上，并且对聚合物的特定表面特征有偏好。其他蛋白质，如哈格曼因子（因子XII），则呈现弥散吸附模式。尚未得到充分研究的具有生理意义的蛋白质，如免疫球蛋白G和因子VIII，很容易吸附到膨体聚四氟乙烯表面。这一发现可能具有重要意义，因为吸附的蛋白质可能激活凝血机制和免疫反应，包括血小板和单核细胞的黏附与激活。利用这项技术可以研究已开发出抗体的任何人类血液蛋白。
