Anderson J M, Ziats N P, Azeez A, Brunstedt M R, Stack S, Bonfield T L
Institute of Pathology, Case Western Reserve University, Cleveland, OH 44106-4907, USA.
J Biomater Sci Polym Ed. 1995;7(2):159-69. doi: 10.1163/156856295x00670.
Static and dynamic human blood adsorption studies on polydimethylsiloxane, PDMS, and silicone rubber show that these materials are similar, but not identical, in their protein adsorption behavior. Fibrinogen, immunoglobulin G, and albumin were the predominant proteins identified on the material surfaces with fibronectin, Hageman factor (factor XII), and factor VIII/vWF adsorbing at intermediate levels. While the protein adsorption characteristics for the two materials were similar, higher levels of the respective proteins were identified on silicone rubber compared to PDMS. Monocytes/macrophages incubated on PDMS, silicone rubber and low density polyethylene, LDPE, with or without protein adsorption produced variable levels of IL-1 beta, IL-6 and TNF-alpha dependent on the polymer and adsorbed protein. PDMS showed lower levels of the cytokines when compared to the polystyrene control and polyethylene. Protein preadsorption on the PDMS, polystyrene, and LDPE surfaces showed lower levels of cytokines when compared to the respective quantities produced with no protein adsorption suggesting a passivating effect by the protein adsorption phenomenon on monocyte/macrophage activation. Preadsorption of IgG, fibrinogen or fibronectin decreased the quantitative expression of IL-1 beta but increased the functional activity in the thymocyte proliferation assay indicating the presence of monocyte/macrophage activation products which either downregulated the activity of IL-1 beta or upregulated thymocyte proliferation in an independent fashion.
对聚二甲基硅氧烷（PDMS）和硅橡胶进行的静态和动态人体血液吸附研究表明，这些材料在蛋白质吸附行为上相似但并不相同。纤维蛋白原、免疫球蛋白G和白蛋白是在材料表面鉴定出的主要蛋白质，纤连蛋白、哈格曼因子（因子XII）和因子VIII/vWF以中等水平吸附。虽然两种材料的蛋白质吸附特性相似，但与PDMS相比，硅橡胶上相应蛋白质的水平更高。在PDMS、硅橡胶和低密度聚乙烯（LDPE）上培养的单核细胞/巨噬细胞，无论有无蛋白质吸附，都会产生不同水平的IL-1β、IL-6和TNF-α，这取决于聚合物和吸附的蛋白质。与聚苯乙烯对照和聚乙烯相比，PDMS显示出较低水平的细胞因子。与无蛋白质吸附时产生的相应量相比，PDMS、聚苯乙烯和LDPE表面的蛋白质预吸附显示出较低水平的细胞因子，这表明蛋白质吸附现象对单核细胞/巨噬细胞激活具有钝化作用。IgG、纤维蛋白原或纤连蛋白的预吸附降低了IL-1β的定量表达，但在胸腺细胞增殖试验中增加了功能活性，这表明存在单核细胞/巨噬细胞激活产物，这些产物要么下调IL-1β的活性，要么以独立方式上调胸腺细胞增殖。
