Division of Infectious Diseases, Tropical Medicine and AIDS, Academic Medical Centre, Amsterdam, The Netherlands.
Clin Microbiol Infect. 2011 Dec;17(12):1823-8. doi: 10.1111/j.1469-0691.2010.03434.x. Epub 2010 Dec 14.
Little is known about polyclonal peripheral blood plasmacytosis in dengue virus (DENV)-infected patients. We initiated this prospective observational study to quantify and describe the kinetics and phenotype of peripheral blood plasma cells (PCs) in these patients. Morphological examination and flow cytometric (FC) analysis for the characterization and immunophenotyping of lymphocyte subsets and PCs were performed in 35 and 31 patients suspected of DENV infection, respectively. Our results show that blood plasmacytosis is a very common haematological finding. Depending on the days of illness at presentation, blood plasmacytosis was observed in 64% to 73% of patients. Blood plasmacytosis was most pronounced before 7 days of illness and declined rapidly thereafter, to completely disappear after 14 days of illness. Blood plasmacytosis was higher in secondary DENV infection. The majority of CD138(+) PCs (89%) had a shared immunophenotype (CD45(+)/CD19(-)/CD56(-)) and in all cases the PCs were polyclonal. Blood plasmacytosis, characterized by a transient presence of polyclonal PCs in the circulation, is a common event in DENV infection.
关于登革热病毒（DENV）感染患者的多克隆外周血浆细胞增多症知之甚少。我们启动了这项前瞻性观察研究，以定量和描述这些患者外周血浆细胞（PC）的动力学和表型。对 35 名疑似 DENV 感染的患者进行了形态学检查和流式细胞术（FC）分析，以对淋巴细胞亚群和 PC 进行特征描述和免疫表型分析。我们的结果表明，血液浆细胞增多症是一种非常常见的血液学发现。根据就诊时的病程天数，64%至 73%的患者出现血液浆细胞增多症。血液浆细胞增多症在疾病的前 7 天最为明显，此后迅速下降，在疾病的第 14 天完全消失。二次 DENV 感染的血液浆细胞增多症更为明显。大多数 CD138(+)PC（89%）具有共享的免疫表型（CD45(+)/CD19(-)/CD56(-)），并且所有情况下的 PC 都是多克隆的。血液浆细胞增多症，表现为循环中短暂存在多克隆 PC，是 DENV 感染的常见事件。
