Division of Infectious Diseases, National Health Research Institutes, Zhunan, Miaoli, Taiwan.
Clin Microbiol Infect. 2011 Sep;17(9):1391-6. doi: 10.1111/j.1469-0691.2010.03436.x. Epub 2010 Dec 22.
The Beijing genotype of Mycobacterium tuberculosis is an endemic lineage in East Asia that has disseminated worldwide. It is a major health concern, as it is geographically widespread and is considered to be hypervirulent. To elucidate its genetic diversity in Taiwan, phylogenetic reconstruction was performed using 338 M. tuberculosis Beijing family clinical isolates. Region-of-difference analysis revealed the strains from Taiwan to be distributed among six subgroups of a phylogenetic tree. Synonymous single nucleotide polymorphisms at 10 chromosomal positions were also analysed. Among the 338 isolates analysed for single-nucleotide polymorphisms by using mass spectrometry, the most frequent strain found was ST10 (53.3%), followed by ST19 (14.8%) and ST22 (14.5%). Tests of drug resistance showed that the sublineages ST10, ST19 and ST26 were over-represented in the multidrug-resistant population. The presence of mutations in putative genes coding for DNA repair enzymes, which could confer a mutator phenotype to facilitate spreading of the pathogen, did not demonstrate an association with multidrug resistance. Therefore, the DNA repair genes may be involved in transmission but not in drug resistance.
结核分枝杆菌北京基因型是东亚地区的地方性谱系，已在全球范围内传播。它是一个主要的健康关注点，因为它在地理上广泛分布，并被认为具有超强的毒力。为了阐明台湾的遗传多样性，使用 338 株结核分枝杆菌北京家族临床分离株进行了系统发育重建。差异区分析显示，来自台湾的菌株分布在系统发育树的六个亚群中。还分析了 10 个染色体位置的同义单核苷酸多态性。在使用质谱法分析 338 个单核苷酸多态性的分离株中，最常见的菌株是 ST10（53.3%），其次是 ST19（14.8%）和 ST22（14.5%）。耐药性测试表明，亚谱系 ST10、ST19 和 ST26 在耐多药人群中过度表达。编码 DNA 修复酶的假定基因中的突变，可能赋予突变体表型以促进病原体的传播，但与耐多药无关。因此，DNA 修复基因可能参与传播，但不参与耐药性。
