Xie Li, Wang Tingting, Yu Shaorong, Chen Xi, Wang Lifeng, Qian Xiaoping, Yu Lixia, Ding Yitao, Zhang Chenyu, Liu Baorui
The Comprehensive Cancer Center of Drum Tower Hospital affiliated to Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjng 21008, PR China; Jiangsu Key Laboratory for Molecular Medicine, Nanjing 210008, PR China.
Clin Biochem. 2011 Feb;44(2-3):216-20. doi: 10.1016/j.clinbiochem.2010.11.002. Epub 2010 Nov 18.
The study was trying to figure out the possible role of cell-free miR-24 and miR-30d as diagnostic biomarkers for malignant effusions.
A total of 110 effusion samples (28 benign and 82 malignant) were collected and cell-free total RNA was extracted. Quantitative RT-PCR was carried out to measure the expression levels of miR-24 and miR-30d, with an exogenous supplemented plant miRNA, ath-miR156a as the reference RNA.
Malignant pleural effusions had higher expression levels of cell-free miR-24 and miR-30d than benign effusions. In the ascites group, only miR-30d was found to be significantly up-regulated in the supernatant of effusions. Combination of cell-free miR-24 and miR-30d could discriminate malignant ascites from benign with improved potency comparing to single miR-30d.
Cell-free miR-30d and miR-24 are potential diagnostic biomarkers for malignant and benign effusions.
本研究旨在探究游离miR - 24和miR - 30d作为恶性积液诊断生物标志物的潜在作用。
共收集110份积液样本（28份良性和82份恶性），提取游离总RNA。采用定量逆转录聚合酶链反应（qRT - PCR）检测miR - 24和miR - 30d的表达水平，以外源补充的植物miRNA，即拟南芥miR156a作为参考RNA。
恶性胸腔积液中游离miR - 24和miR - 30d的表达水平高于良性积液。在腹水组中，仅发现miR - 30d在积液上清液中显著上调。与单一miR - 30d相比，游离miR - 24和miR - 30d联合使用能够更有效地鉴别恶性腹水和良性腹水。
游离miR - 30d和miR - 24是恶性和良性积液的潜在诊断生物标志物。
