Liu Chongmei, Huang Liuyan, Zhang Xuechun, Yang Juan
Department of Pathology, Yueyang Second People's Hospital, Yueyang, Hunan, China.
Graduate School of The University of South China, Hengyang, Hunan, China.
Interact Cardiovasc Thorac Surg. 2018 Mar 1;26(3):376-381. doi: 10.1093/icvts/ivx327.
The study aimed to assess the combination of DNA ploidy analysis (DPA) and the expression of microRNA-21 (miR-21) or microRNA-24 (miR-24) in the detection of malignant pleural effusion (MPE).
In this prospective research, a total of 40 samples (20 benign and 20 malignant effusions), flexural effusion exfoliated cells and cell-free miR-21 and miR-24 were collected. DPA and exfoliative cytology examinations were conducted to diagnose flexural effusion exfoliated cells. Quantitative reverse transcriptase polymerase chain reaction was carried out to measure the expressions of miR-21 and miR-24. Receiver operating characteristic curve and the area under the curve were applied to evaluate the accuracy rate of different diagnostic approaches on MPE.
In the MPE group, DPA demonstrated a higher rate of accuracy in MPE diagnosis than exfoliative cytology. The expressions of miR-21 and miR-24 were significantly higher in MPE than in benign pleural effusion (P < 0.05). Furthermore, area under the curve, sensitivity and specificity were 0.942, 95% and 90% for the combination of miR-21 and DPA and 0.973, 100% and 80% for the union of miR-24 and DPA, respectively, representing a significant improvement in both accuracy and sensitivity. Therefore, the combination of DPA and miR-21 or miR-24 appears to be a better biomarker for discriminating MPE from benign pleural effusion.
The combination of DPA and miR-21 or miR-24 may function as a promising diagnostic tool of MPE.
ChiCTR-TRC-14004719.
本研究旨在评估DNA倍体分析（DPA）与微小RNA-21（miR-21）或微小RNA-24（miR-24）的表达在恶性胸腔积液（MPE）检测中的联合应用。
在这项前瞻性研究中，共收集了40份样本（20份良性积液和20份恶性积液）、胸腔积液脱落细胞以及游离的miR-21和miR-24。进行DPA和脱落细胞学检查以诊断胸腔积液脱落细胞。采用定量逆转录聚合酶链反应来测量miR-21和miR-24的表达。应用受试者工作特征曲线及曲线下面积来评估不同诊断方法对MPE的准确率。
在MPE组中，DPA在MPE诊断中的准确率高于脱落细胞学。MPE中miR-21和miR-24的表达显著高于良性胸腔积液（P＜0.05）。此外，miR-21与DPA联合的曲线下面积、敏感性和特异性分别为0.942、95%和90%，miR-24与DPA联合的分别为0.973、100%和80%，在准确性和敏感性方面均有显著提高。因此，DPA与miR-21或miR-24联合似乎是鉴别MPE与良性胸腔积液的更好生物标志物。
DPA与miR-21或miR-2联合可能是一种有前景的MPE诊断工具。
ChiCTR-TRC-14004719。
