Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Exp Parasitol. 2011 Feb;127(2):581-6. doi: 10.1016/j.exppara.2010.11.002. Epub 2010 Nov 17.
Increasing evidence indicates miRNAs as critical regulators of gene expression, but little information is available for miRNAs in intestinal diseases. Here, we investigated intestinal infections of male Balb/c mice with the coccidian parasite Eimeria papillata. On day 4 after oral infection, mice were shedding 3150 ± 430 oocysts per gram feces. This was associated with a low inflammatory response of the jejunum of mice evidenced by histology, non-response of IL-1β mRNA, even slight downregulation of IL-6 mRNA, only slight increases in iNOS mRNA, nitrate/nitrate, malondialdehyde, and a small decrease in glutathione, respectively. Only IFNγ mRNA was strongly induced. Using miRNA microarray technology, there were significantly upregulated the four miRNA species miR-1959, MCMV-miR-M23-1-5P, miR-203, and miR-21 out of 634 miRNAs, which was also confirmed by quantitative RT-PCR. Our data provide evidence that E. papillata parasites are able to induce specific miRNA species in their host target organ.
越来越多的证据表明 miRNAs 是基因表达的关键调节因子，但关于肠道疾病中的 miRNAs 信息却很少。在这里，我们研究了雄性 Balb/c 小鼠感染小囊虫寄生虫 Eimeria papillata 的肠道感染。在口服感染后的第 4 天，小鼠每克粪便中排出 3150±430 个卵囊。这与小鼠空肠的炎症反应较低有关，这一点可以从组织学上得到证明，即 IL-1β mRNA 没有反应，甚至 IL-6 mRNA 略有下调，只有 iNOS mRNA 略有增加，硝酸盐/亚硝酸盐、丙二醛略有增加，谷胱甘肽略有减少。只有 IFNγ mRNA 被强烈诱导。使用 miRNA 微阵列技术，在 634 种 miRNA 中，有 4 种 miRNA 物种（miR-1959、MCMV-miR-M23-1-5P、miR-203 和 miR-21）显著上调，这也通过定量 RT-PCR 得到了证实。我们的数据提供了证据表明，E. papillata 寄生虫能够在其宿主靶器官中诱导特定的 miRNA 物种。
