Department of Biochemistry, La Trobe University, Bundoora, Victoria 3086, Australia.
J Microbiol Methods. 2011 Jan;84(1):121-7. doi: 10.1016/j.mimet.2010.11.010. Epub 2010 Nov 18.
Canola is second only to soybean as the most important oilseed crop in the world. The global production of canola is forecast to continue to increase and as a result the canola industry will continue to flourish. However, it is threatened by several fungal diseases that affect canola and cost producers hundreds of millions of dollars a year in reduced yield and quality. Blackleg is the most common and devastating disease of canola and is caused by the fungus Leptosphaeria maculans. The fungus can infect any part of the plant at all growth stages and is a serious threat to the canola industry. Novel and more efficient antifungal agents which interfere with fungal growth and development are clearly needed to control this pathogen. This paper reports the establishment of a simple functional assay system for the screening of antifungal proteins against a virulent strain of L. maculans.
油菜籽是世界上仅次于大豆的第二重要的油料作物。全球油菜籽产量预计将继续增加，因此油菜籽产业将继续繁荣。然而，它受到几种影响油菜籽的真菌病的威胁，这些疾病每年导致生产者减产和降低质量，损失数亿美元。黑胫病是油菜籽最常见和最具破坏性的疾病，由真菌菜豆壳球腔菌引起。该真菌可以在植物的所有生长阶段感染任何部位，对油菜籽产业构成严重威胁。显然，需要新型且更有效的抗真菌剂来干扰真菌的生长和发育，以控制这种病原体。本文报告了一种针对菜豆壳球腔菌强毒株筛选抗真菌蛋白的简单功能测定系统的建立。
