Oberdick J, Smeyne R J, Mann J R, Zackson S, Morgan J I
Department of Neuroscience, Roche Research Center, Nutley, NJ 07110.
Science. 1990 Apr 13;248(4952):223-6. doi: 10.1126/science.2109351.
A genomic clone encoding the Purkinje cell-specific L7 protein has been isolated and utilized to drive the expression of beta-galactosidase in mice. Three independent transgenic lines, germ line transformed with an L7-beta-galactosidase fusion gene, exhibit beta-galactosidase expression in both cerebellar Purkinje cells and retinal bipolar neurons. This distribution is the same as that previously determined for the L7 protein by immunohistochemistry. The transgenic murine lines can be used to obtain populations of marked Purkinje and bipolar neurons. Similar L7 promoter constructs can be used to express other foreign genes specifically in these two classes of neurons.
一个编码浦肯野细胞特异性L7蛋白的基因组克隆已被分离出来，并用于驱动小鼠体内β-半乳糖苷酶的表达。三个独立的转基因品系，用L7-β-半乳糖苷酶融合基因进行种系转化，在小脑浦肯野细胞和视网膜双极神经元中均表现出β-半乳糖苷酶的表达。这种分布与先前通过免疫组织化学确定的L7蛋白分布相同。转基因小鼠品系可用于获得标记的浦肯野细胞和双极神经元群体。类似的L7启动子构建体可用于在这两类神经元中特异性表达其他外源基因。
