Division of Bioengineering, National University of Singapore, Singapore.
Biosens Bioelectron. 2011 Jan 15;26(5):2436-41. doi: 10.1016/j.bios.2010.10.028. Epub 2010 Oct 23.
In this study, we present a portable and generic DNA bioassay system based on in situ oligonucleotide synthesis followed by hybridization based detection. The system include two main parts, an oligonucleotide synthesizer and a fluorescence detection system. The oligonucleotide synthesizer is based on microfluidic technology and capable of synthesizing any desired oligonucleotide which can be either used as a primer for PCR based detection (external) or a probe for hybridization based detection (integrated) of a target DNA analyte. The oligonucleotide sequence can be remotely sent to the system. The integrated fluorescence detection system is based on a photodiode to detect Texas Red fluorophore as low as 0.5 fmol. The complete system, integrating the oligonucleotide synthesizer and fluorescence detection system, was successfully used to distinguish DNA from two different bacteria strains. The presented generic portable instrument has the potential to detect any desired DNA target sequence in the field. Potential applications are for homeland security and fast responses to emerging bio-threats.
在本研究中，我们提出了一种基于原位寡核苷酸合成和杂交检测的便携式通用 DNA 生物分析系统。该系统包括两个主要部分，即寡核苷酸合成器和荧光检测系统。寡核苷酸合成器基于微流控技术，能够合成任何所需的寡核苷酸，这些寡核苷酸既可以用作基于 PCR 检测的引物（外部），也可以用作目标 DNA 分析物杂交检测的探针（集成）。寡核苷酸序列可以远程发送到系统。集成的荧光检测系统基于光电二极管，可检测低至 0.5 fmol 的 Texas Red 荧光染料。完整的系统，即寡核苷酸合成器和荧光检测系统的集成，成功地用于区分两种不同细菌菌株的 DNA。所提出的通用便携式仪器具有在现场检测任何所需 DNA 目标序列的潜力。潜在的应用是用于国土安全和对新兴生物威胁的快速响应。
