Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt.
Bioresour Technol. 2011 Feb;102(3):2387-93. doi: 10.1016/j.biortech.2010.10.106. Epub 2010 Oct 28.
Biodegradation of chicken feathers waste directed by Bacillus subtilis DB 100 (p5.2) cells was successfully carried out in 14L Bio Flo 110 laboratory scale fermentor. Seven liters of feathers-based modified basal medium II, feathers-based tap water and feathers-based distilled water separately in the fermentor were inoculated with activated bacterial cells. The fermentation processes were conducted at 37°C, 700 rpm agitation speed and 0.7 vvm air flow rate in the absence of kanamycin. Highest net levels of released feathers hydrolysis end products [soluble proteins and NH(2)-free amino groups] and keratinolytic alkaline protease activity in the fermentor were greatly comparable to those of shake flasks. Interestingly, the plasmid (p5.2) inside the recombinant B. subtilis cells growing in the fermentor displayed 100% stability till the fifth day of incubation and this presents a great challenge. Data certainly would encourage the transfer to larger scale fermentors to carry out feathers biodegradation process.
枯草芽孢杆菌 DB 100（p5.2）细胞定向生物降解鸡毛废弃物在 14L BioFlo110 实验室规模发酵罐中成功进行。7L 基于鸡毛的改良基础培养基 II、基于鸡毛的自来水和基于鸡毛的蒸馏水分别在发酵罐中用活性细菌细胞接种。发酵过程在 37°C、700rpm 搅拌速度和 0.7vvm 空气流速下进行，且不添加卡那霉素。发酵罐中释放的羽毛水解末端产物[可溶性蛋白质和 NH2-游离氨基酸]和角蛋白酶活性的净水平与摇瓶中非常相似。有趣的是，在发酵罐中生长的重组枯草芽孢杆菌细胞内的质粒（p5.2）在孵育的第五天仍保持 100%的稳定性，这是一个巨大的挑战。这些数据肯定会鼓励向更大规模的发酵罐转移以进行羽毛生物降解过程。
