Laboratório de Farmacognosia, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café s/n, Ribeirão Preto, SP, Brazil.
J Pharm Biomed Anal. 2011 Mar 25;54(4):653-9. doi: 10.1016/j.jpba.2010.10.006. Epub 2010 Oct 16.
Copaifera species (Leguminoseae) are popularly known as "copaiba" or "copaíva". The oleoresins obtained from the trunk of these species have been extensively used in folk medicine and are commercialized in Brazil as crude oil and in several pharmaceutical and cosmetic products. This work reports a complete validated method for the quantification of β-caryophyllene, α-copaene, and α-humulene in distinct copaiba oleoresins available commercially. Thus, essential oil samples (100μL) were dissolved in 20mL of hexanes containing internal standard (1,2,4,5-tetramethylbenzene, 3.0mM) in a 25mL glass flask. A 1μL aliquot was injected into the GC-FID system. A fused-silica capillary column HP-5, coated with 5% phenyl-methylsiloxane was used for this study. The developed method gave a good detection response with linearity in the range of 0.10-18.74mM. Limits of detection and quantitation variety ranged between 0.003 and 0.091mM. β-Caryophyllene, α-copaene, and α-humulene were recovered in a range from 74.71% to 88.31%, displaying RSD lower than 10% and relative errors between -11.69% and -25.30%. Therefore, this method could be considered as an analytical tool for the quality control of different Copaifera oil samples and its products in both cosmetic and pharmaceutical companies.
巴西橡胶树（豆科）俗称“巴西橡胶树”或“巴西橡胶树”。从这些物种的树干中获得的油树脂在民间医学中得到了广泛应用，并在巴西作为粗油商业化，并在几种制药和化妆品产品中商业化。本工作报道了一种用于定量分析不同市售巴西橡胶树油树脂中 β-石竹烯、α-古巴烯和α-葎草烯的完整验证方法。因此，将精油样品（100μL）溶解在含有内标（1,2,4,5-四甲基苯，3.0mM）的 20mL 己烷中，将 1μL 等分试样注入 GC-FID 系统。使用涂有 5%苯基-甲基硅氧烷的熔融硅毛细管柱 HP-5 进行这项研究。所开发的方法具有良好的检测响应，在线性范围内为 0.10-18.74mM。检测限和定量范围在 0.003 和 0.091mM 之间。β-石竹烯、α-古巴烯和α-葎草烯的回收率在 74.71%至 88.31%之间，显示出低于 10%的 RSD 和-11.69%至-25.30%之间的相对误差。因此，该方法可以被认为是控制不同巴西橡胶树油样品及其在化妆品和制药公司中的产品质量的分析工具。
