Long Ruiqi, McShane Mike
Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843, USA.
Annu Int Conf IEEE Eng Med Biol Soc. 2010;2010:1033-6. doi: 10.1109/IEMBS.2010.5628059.
Ratiometric Luminescent microparticle sensors have been developed for sensing biochemical targets such as glucose in interstitial fluid, enabling use of dermal implants for on-demand monitoring. For these sensor systems to be deployed in vivo, a matched optoelectronic system for interrogation of dermally-implanted sensors was previously designed, constructed, and evaluated experimentally. During evaluation experiments, it revealed that the system efficiency was compromised by losses due to fiber connections, the entrance aperture, and the entrance slit of the spectrometer. In this work, two optimization methods were investigated to overcome photon loss at fiber connections and internal trade-off between resolution and input light power of the current spectrometer: 1) Replacement of the CCD spectrometer with a two-detector system, enabling extraction of key spectral information by integrating signals over two wavelength regions (reference and sensing emission peaks); and 2) Free-space coupling of the optical probe to a custom low-resolution spectrometer. Photon loss was evaluated by experiments and simulations, preliminary hardware of two-detector system was constructed, and optimization simulations were performed to explore conceptual feasibility of the free-space coupling custom-designed spectrometer.
已开发出比率发光微粒传感器，用于检测诸如间质液中葡萄糖等生化目标，从而能够使用真皮植入物进行按需监测。为了将这些传感器系统部署在体内，先前设计、构建并通过实验评估了一种用于询问真皮植入传感器的匹配光电系统。在评估实验过程中发现，由于光纤连接、光谱仪的入射孔径和入射狭缝造成的损耗，系统效率受到了影响。在这项工作中，研究了两种优化方法，以克服光纤连接处的光子损失以及当前光谱仪分辨率与输入光功率之间的内部权衡：1）用双探测器系统替代电荷耦合器件（CCD）光谱仪，通过在两个波长区域（参考发射峰和传感发射峰）上积分信号来提取关键光谱信息；2）将光学探头自由空间耦合到定制的低分辨率光谱仪。通过实验和模拟评估了光子损失，构建了双探测器系统的初步硬件，并进行了优化模拟，以探索自由空间耦合定制光谱仪的概念可行性。
