Long Ruiqi, McShane Mike
Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843, USA.
Annu Int Conf IEEE Eng Med Biol Soc. 2009;2009:122-5. doi: 10.1109/IEMBS.2009.5334722.
Dermally-implanted microparticle sensors are being developed for on-demand monitoring of blood sugar levels. For these to be deployed in vivo, a matched optoelectronic system for delivery of excitation, collection and analysis of escaping fluorescent signal is needed. Previous studies predicted the characteristics of fluorescence from microparticle sensors to facilitate design of hardware system. Based on the results of simulations, we designed and constructed the optical part of this opto-electronic system. This study experimentally verified the simulation results and tested the capability of the designed optical system. Reliable skin phantoms sufficient for future dynamic tests were developed. Skin phantoms with different thicknesses were made and the optical properties of skin phantoms were determined with an integrating sphere system and Inverse Adding-Doubling method. Measurements of sensor emission spectrum through phantoms with different thicknesses were done with the designed optical system. Simulations for the experiment situation were performed. The experimental measurements agreed well with simulations in most cases. The results of hardware experiment and validation with skin phantoms provided us with critical information for future dynamic tests and animal experiments.
正在开发用于按需监测血糖水平的皮下植入式微粒传感器。为了将这些传感器部署在体内，需要一个匹配的光电系统来传输激发光、收集并分析逸出的荧光信号。先前的研究预测了微粒传感器的荧光特性，以促进硬件系统的设计。基于模拟结果，我们设计并构建了该光电系统的光学部分。本研究通过实验验证了模拟结果，并测试了所设计光学系统的性能。开发了足以用于未来动态测试的可靠皮肤模型。制作了不同厚度的皮肤模型，并用积分球系统和反向倍增法测定了皮肤模型的光学特性。使用所设计的光学系统对通过不同厚度模型的传感器发射光谱进行了测量。对实验情况进行了模拟。在大多数情况下，实验测量结果与模拟结果吻合良好。硬件实验结果以及使用皮肤模型进行的验证为我们未来的动态测试和动物实验提供了关键信息。
