Long Ruiqi, McShane Mike
Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843, USA.
Annu Int Conf IEEE Eng Med Biol Soc. 2012;2012:2351-4. doi: 10.1109/EMBC.2012.6346435.
Ratiometric luminescent microparticle sensors have been developed for sensing biochemical targets such as glucose in interstitial fluid, enabling use of dermal implants for on-demand monitoring. For these sensor systems to be deployed in vivo, a matched optoelectronic system for interrogation of dermally-implanted sensors was previously designed, constructed, and evaluated experimentally. During evaluation experiments, it revealed that the system efficiency was compromised by losses due to fiber connections of a commercial spectrometer. In this work, a high-throughput spectral system was presented to solve the photon loss problem. This system was designed, constructed, and tested. The throughput was around hundred time more than the previous system we used, and it was cost-effective, as well. It enables use of an integrated system for excitation, collection and measurement of luminescent emission, and will be used as a tool for in vivo studies with animal models or human subjects.
已经开发出比率发光微粒传感器来检测生化目标，如组织间液中的葡萄糖，从而能够使用真皮植入物进行按需监测。为了将这些传感器系统部署在体内，之前设计、构建并通过实验评估了一个用于询问真皮植入传感器的匹配光电系统。在评估实验期间，发现由于商业光谱仪的光纤连接导致的损耗降低了系统效率。在这项工作中，提出了一种高通量光谱系统来解决光子损失问题。该系统经过设计、构建和测试。其通量比我们之前使用的系统高出约一百倍，而且具有成本效益。它能够使用一个集成系统来激发、收集和测量发光发射，并将用作动物模型或人类受试者体内研究的工具。
