Besson F, Chevanet C, Michel G
Laboratoire de Biochimie Microbienne, Université Claude Bernard, Lyon I, Villeurbanne, France.
J Gen Microbiol. 1987 Mar;133(3):767-72. doi: 10.1099/00221287-133-3-767.
The production of iturin A by Bacillus subtilis was studied with respect to the composition of the culture medium. Increasing phosphate concentrations did not modify the antibiotic yield. Fructose, sucrose and mannitol were better carbon sources than glucose for antibiotic production. The nature of the nitrogen source was an important factor in the production of antibiotic. Among the amino acids which are components of iturin A, L-asparagine was the best substrate for the biosynthesis of iturin A; L-glutamine and L-serine were rather poor substrates while L-proline and D-tyrosine gave no antibiotic. Ammonium salts permitted good synthesis of antibiotic but the addition of calcium ions to the culture medium inhibited the excretion of antibiotic from the cells.
研究了枯草芽孢杆菌产伊枯草菌素A与培养基成分之间的关系。提高磷酸盐浓度并不会改变抗生素产量。对于抗生素生产而言，果糖、蔗糖和甘露醇是比葡萄糖更好的碳源。氮源的性质是抗生素生产中的一个重要因素。在构成伊枯草菌素A的氨基酸中，L-天冬酰胺是伊枯草菌素A生物合成的最佳底物；L-谷氨酰胺和L-丝氨酸是较差的底物，而L-脯氨酸和D-酪氨酸则不产生抗生素。铵盐有利于抗生素的良好合成，但向培养基中添加钙离子会抑制抗生素从细胞中的排泄。
