Department of Histology and Embryology, Medical University, Chalubinskiego 6a, 50-368 Wroclaw, Poland.
Anticancer Res. 2012 Mar;32(3):773-7.
Investigation of the relationship between expression of Ki-67 antigen and minichromosome maintenance 2 (MCM-2) protein by using an immunohistochemical study on whole sections (WS) and sections of invasive ductal breast cancer (IDC) obtained in the tissue microarray (TMA) technique.
Material consisted of 51 archival paraffin blocks of IDC. Tissue microarrays were composed of 0.6 mm core punches. Reactions were performed using specific antibodies (anti-Ki-67 and anti-MCM-2). Intensity of the marker expression was evaluated using computer-assisted image analysis. For statistical purposes, three different tests were used.
Spearman rank correlation revealed a strong positive correlation between expression of tested markers: Ki-67 (TMA) vs. Ki-67 (WS) (r=0.91, p<0.05) and MCM-2 (TMA) vs. MCM-2 (WS) (r=0.87, p<0.05). Mann Whitney U-test showed no significant differences between the two markers in both analysed techniques. Moreover, the Bland-Altman plot demonstrated a low level of bias between the analysed methods.
This study shows that TMA could have a great potential in evaluation of proliferative activity in IDC and that MCM-2 protein might be a specific and sensitive marker of cell proliferation.
通过使用组织微阵列（TMA）技术获得的全切片（WS）和浸润性导管乳腺癌（IDC）切片，研究 Ki-67 抗原和微小染色体维持蛋白 2（MCM-2）的表达之间的关系。
材料包括 51 例 IDC 的存档石蜡块。组织微阵列由 0.6mm 核心打孔器组成。使用特异性抗体（抗 Ki-67 和抗 MCM-2）进行反应。使用计算机辅助图像分析评估标记物表达的强度。出于统计目的，使用了三种不同的测试。
Spearman 等级相关显示，测试标记物之间存在强烈的正相关：Ki-67（TMA）与 Ki-67（WS）（r=0.91，p<0.05）和 MCM-2（TMA）与 MCM-2（WS）（r=0.87，p<0.05）。Mann-Whitney U 检验显示两种标记物在两种分析技术中均无显著差异。此外，Bland-Altman 图表明两种分析方法之间的偏差水平较低。
本研究表明，TMA 可能在评估 IDC 的增殖活性方面具有巨大潜力，并且 MCM-2 蛋白可能是细胞增殖的特异性和敏感标志物。
