Department of Chemical Engineering, Pennsylvania State University, University Park, PA 16802, USA.
Appl Environ Microbiol. 2011 Jan;77(2):706-9. doi: 10.1128/AEM.01890-10. Epub 2010 Nov 19.
Anaerobic glucose oxidation was coupled to xylose reduction in a nonfermentative Escherichia coli strain expressing NADPH-dependent xylose reductase. Xylitol production serves as the primary means of NAD(P)(+) regeneration, as glucose is converted primarily to acetate and CO(2). The membrane-bound transhydrogenase PntAB is required to achieve the maximum theoretical yield of four moles of xylitol per mole of glucose consumed.
在表达 NADPH 依赖型木糖还原酶的非发酵型大肠杆菌菌株中，无氧葡萄糖氧化与木糖还原偶联。木糖醇的生产是 NAD(P)(+) 再生的主要手段，因为葡萄糖主要转化为乙酸盐和 CO(2)。需要膜结合的转氢酶 PntAB 才能实现每消耗 1 摩尔葡萄糖产生 4 摩尔木糖醇的最大理论产率。
