大肠杆菌中不存在天然发酵途径时的厌氧必需木糖醇生产。
Anaerobic obligatory xylitol production in Escherichia coli strains devoid of native fermentation pathways.
机构信息
Department of Chemical Engineering, Pennsylvania State University, University Park, PA 16802, USA.
出版信息
Appl Environ Microbiol. 2011 Jan;77(2):706-9. doi: 10.1128/AEM.01890-10. Epub 2010 Nov 19.
Abstract
Anaerobic glucose oxidation was coupled to xylose reduction in a nonfermentative Escherichia coli strain expressing NADPH-dependent xylose reductase. Xylitol production serves as the primary means of NAD(P)(+) regeneration, as glucose is converted primarily to acetate and CO(2). The membrane-bound transhydrogenase PntAB is required to achieve the maximum theoretical yield of four moles of xylitol per mole of glucose consumed.
摘要
在表达 NADPH 依赖型木糖还原酶的非发酵型大肠杆菌菌株中，无氧葡萄糖氧化与木糖还原偶联。木糖醇的生产是 NAD(P)(+) 再生的主要手段，因为葡萄糖主要转化为乙酸盐和 CO(2)。需要膜结合的转氢酶 PntAB 才能实现每消耗 1 摩尔葡萄糖产生 4 摩尔木糖醇的最大理论产率。
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