在大肠杆菌中构建异丁醇生产的代谢途径。
Reconstruction of metabolic pathway for isobutanol production in Escherichia coli.
机构信息
Center for Sustainable Resource Science, RIKEN, 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan.
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan.
出版信息
Microb Cell Fact. 2019 Jul 18;18(1):124. doi: 10.1186/s12934-019-1171-4.
BACKGROUND
The microbial production of useful fuels and chemicals has been widely studied. In several cases, glucose is used as the raw material, and almost all microbes adopt the Embden-Meyerhof (EM) pathway to degrade glucose into compounds of interest. Recently, the Entner-Doudoroff (ED) pathway has been gaining attention as an alternative strategy for microbial production.
RESULTS
In the present study, we attempted to apply the ED pathway for isobutanol production in Escherichia coli because of the complete redox balance involved. First, we generated ED pathway-dependent isobutanol-producing E. coli. Thereafter, the inactivation of the genes concerning organic acids as the byproducts was performed to improve the carbon flux to isobutanol from glucose. Finally, the expression of the genes concerning the ED pathway was modified.
CONCLUSIONS
The optimized isobutanol-producing E. coli produced 15.0 g/L of isobutanol as the final titer, and the yield from glucose was 0.37 g/g (g-glucose/g-isobutanol).
背景
微生物生产有用燃料和化学品的研究已经很广泛。在某些情况下，葡萄糖被用作原料，几乎所有的微生物都采用 EMP 途径将葡萄糖降解为感兴趣的化合物。最近，ED 途径作为微生物生产的替代策略受到关注。
结果
在本研究中，由于涉及完整的氧化还原平衡，我们试图在大肠杆菌中应用 ED 途径生产异丁醇。首先，我们生成了依赖 ED 途径的产异丁醇大肠杆菌。然后，失活与有机酸等副产物相关的基因，以提高葡萄糖向异丁醇的碳通量。最后，对 ED 途径相关基因的表达进行了修饰。
结论
优化后的产异丁醇大肠杆菌最终产生了 15.0 g/L 的异丁醇，葡萄糖得率为 0.37 g/g（g-葡萄糖/g-异丁醇）。
Zotero 插件