Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina.
Antimicrob Agents Chemother. 2011 Feb;55(2):917-20. doi: 10.1128/AAC.00491-10. Epub 2010 Nov 22.
ISAba825, an insertion sequence found inactivating Acinetobacter baumannii carO, was tagged with a kanamycin (Kn) resistance cassette. ISAba825::Kn effectively transposed in A. baumannii, showing preference for short, AT-enriched target sequences, generating 6- to 9-bp target duplications. Additionally, we detected the presence of ISAba825 upstream of a plasmid-borne bla(OXA-58) gene, generating a hybrid promoter largely enhancing its expression and leading to carbapenem resistance. Overall, a role for ISAba825 in carbapenem resistance modulation in A. baumannii is proposed.
ISAba825 是一种插入序列，可使鲍曼不动杆菌的 carO 失活，该序列被带有卡那霉素（Kn）抗性盒的标签。ISAba825::Kn 有效地在鲍曼不动杆菌中转座，表现出对短而富含 AT 的靶序列的偏好，产生 6-9bp 的靶序列重复。此外，我们还检测到 ISAba825 位于质粒携带的 bla（OXA-58）基因上游，产生了一个混合启动子，大大增强了其表达，导致碳青霉烯类药物耐药。总的来说，我们提出了 ISAba825 在鲍曼不动杆菌碳青霉烯类药物耐药性调节中的作用。
