Insights into the complete genomes of carbapenem-resistant harbouring and genes using a hybrid-assembly approach.

Carbapenem resistance in is due to , which is endemic in India. Recently, the sporadic presence of as well as the occurrence of dual carbapenemases were observed. The mobility as well as the dissemination of these resistance genes were mainly mediated by various mobile genetic elements. The present study was aimed at characterizing the genetic arrangement of and identified in two complete genomes of carbapenem-resistant (CRAB). Complete genomes obtained using a hybrid-assembly approach revealed the accurate arrangement of Tn with IS with and IS with In addition, the association of 1 integrase with the gene and several virulence factors required for type-IV pili assembly, motility and biofilm formation have been identified. The current study provided deeper insight into the complete characterization of insertion sequences and transposons associated with the carbapenem-resistant genes using short reads of IonTorrent PGM and long reads of MinIon in .