Department of Dermatology, Leiden University Medical Center, Leiden, the Netherlands.
Cancer Res. 2010 Dec 1;70(23):9777-86. doi: 10.1158/0008-5472.CAN-10-0352. Epub 2010 Nov 23.
Human papillomaviruses (betaPV) from the beta genus cannot be classified according to their oncogenicity due to a paucity of information. This study evaluates the association between betaPV infection and cutaneous squamous cell carcinoma in conjunction with measures of UV exposure and susceptibility. We performed case-control studies in the Netherlands, Italy, and Australia, countries with profoundly different UV exposures. The presence of 25 betaPV types in eyebrow hair follicles was determined using a highly sensitive HPV DNA genotyping assay, and antibodies for the 15 most prevalent betaPV types in a total of 689 squamous cell carcinoma cases and 845 controls were detected using multiplex serology. Multivariate logistic regression models were used for case-control comparisons and interaction analyses. BetaPV DNA was detected in eyebrow hairs of more than 90% of all participants. The presence of betaPV DNA was associated with an increased risk of squamous cell carcinoma in the Netherlands (OR = 2.8; 95% CI 1.3-5.8) and Italy (OR = 1.7; 95% CI 0.79-3.6), but not in Australia (OR = 0.91; 95% CI 0.53-1.6). Seropositivity for betaPV in controls ranged between 52% and 67%. A positive antibody response against 4 or more betaPV types was associated with squamous cell carcinoma in Australia (OR = 2.2; 95% CI 1.4-3.3), the Netherlands (OR = 2.0; 95% CI 1.2-3.4) and fair-skinned Italians (OR = 1.6, 95% CI 0.94- 2.7). The association between UV susceptibility and squamous cell carcinoma was stronger in betaPV-seropositive people. These combined data support the hypothesis that betaPV may play a role in the development of cutaneous squamous cell carcinoma.
人乳头瘤病毒（βPV）β 属不能根据其致癌性进行分类，因为信息不足。本研究评估了βPV 感染与皮肤鳞状细胞癌之间的关联，同时考虑了紫外线暴露和易感性的措施。我们在荷兰、意大利和澳大利亚进行了病例对照研究，这些国家的紫外线暴露程度差异很大。使用高度敏感的 HPV DNA 基因分型检测，在 689 例鳞状细胞癌病例和 845 例对照者的眉毛毛囊中检测到 25 种βPV 型；使用多重血清学检测总共检测到 15 种最常见的βPV 型的抗体。使用多变量逻辑回归模型进行病例对照比较和交互分析。超过 90%的所有参与者的眉毛毛发中都检测到βPV DNA。βPV DNA 的存在与荷兰（OR=2.8；95%CI 1.3-5.8）和意大利（OR=1.7；95%CI 0.79-3.6）鳞状细胞癌的风险增加相关，但在澳大利亚（OR=0.91；95%CI 0.53-1.6）不相关。对照组中βPV 的血清阳性率在 52%至 67%之间。对 4 种或更多βPV 型的抗体阳性反应与澳大利亚（OR=2.2；95%CI 1.4-3.3）、荷兰（OR=2.0；95%CI 1.2-3.4）和白种意大利人（OR=1.6，95%CI 0.94-2.7）的鳞状细胞癌相关。在βPV 血清阳性者中，紫外线易感性与鳞状细胞癌之间的相关性更强。这些综合数据支持βPV 可能在皮肤鳞状细胞癌的发生中起作用的假说。
